Cytogenetic studies: Premature chromosome condensation (PCC) in peripheral blood lymphocytes cultures and skin warts lesions cultures induced by Papillomavirus spontaneous infection in bovines

Abstract

Abstract Papillomavirus is a dermotropic oncogenic DNA virus which induces benign and malignant lesions described in a wide variety of hosts. Evidences suggest that the virus is species and tissue specific. However several reports have shown the development of lesions in phylogenetically close hosts. We analyzed a total of 30 blood and tumors samples from bovines affected cutaneous papillomatosis lesions. Our study reported premature chromosome condensation (PCC) in peripheral blood lymphocytes cultures and in skin warts cells cultures, and we detected epithelial cellular hyperproliferation confirmed by histological tissues processing. The morphology of the PCC reflects the cell cycle phase-specific exhibiting levels of the prematurely condensed chromatin. These results indicated a failed in the cellular control mechanism with disruption of mitotic checkpoints induced by virus spontaneous infection.