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https://repositorio.ufba.br/handle/ri/13682
metadata.dc.type: | Artigo de Periódico |
Title: | Azurocidin, a Natural Antibiotic from Human Neutrophils: Expression, Antimicrobial Activity, and Secretion |
Other Titles: | Protein Expression and Purification |
Authors: | Almeida, Roque Pacheco de Vanet, Anne Witko-Sarsat, Veronique Melchior, Maxine McCabe, Denise Gabay, Joelle E. |
metadata.dc.creator: | Almeida, Roque Pacheco de Vanet, Anne Witko-Sarsat, Veronique Melchior, Maxine McCabe, Denise Gabay, Joelle E. |
Abstract: | The azurophil granules of human PMN contain four antibiotic proteins, the serprocidins, which have extensive homology to one another and to serine proteases. Azurocidin, a member of this family, is a 29-kDa glycoprotein with broad spectrum antimicrobial activity and chemotactic activity toward monocytes. Insect cells transfected with a baculovirus vector carrying azurocidin cDNA produced a recombinant azurocidin protein. We purified the recombinant azurocidin protein from the culture medium of the infected cells and showed that it retained the antimicrobial activity of the native neutrophil-derived molecule. In addition, we present evidence that a 49-amino-acid region of the recombinant azurocidin protein is required for its secretion from insect cells. |
Publisher: | Protein Expression and Purification |
metadata.dc.rights: | Acesso Aberto |
URI: | http://repositorio.ufba.br/ri/handle/ri/13682 |
Issue Date: | 1996 |
Appears in Collections: | Artigo Publicado em Periódico (Faculdade de Medicina) |
Files in This Item:
File | Description | Size | Format | |
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11111111.pdf_0 | 365,31 kB | Adobe PDF | View/Open |
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