Por favor, use este identificador para citar o enlazar este ítem:
https://repositorio.ufba.br/handle/ri/7459
metadata.dc.type: | Artigo de Periódico |
Título : | Effect of different lectins on the proliferation of goat peripheral blood mononuclear cells |
Otros títulos : | Small Ruminant Research |
Autor : | Regis, Lia Fernandes Ribeiro, Marcos Vale, Vera Paule, Bruno Nascimento, Ivana Lúcia de Oliveira Schaer, Robert Eduard Nascimento, Roberto José Meyer Freire, Songeli |
metadata.dc.creator: | Regis, Lia Fernandes Ribeiro, Marcos Vale, Vera Paule, Bruno Nascimento, Ivana Lúcia de Oliveira Schaer, Robert Eduard Nascimento, Roberto José Meyer Freire, Songeli |
Resumen : | In the present study we determined the effect of the lectins concanavalin A (Con A), phytohemagglutinin (PHA) and pokeweed (PWM), frequently used in cell proliferation assays, on the proliferation of goat peripheral blood mononuclear cells (PBMCs). The use of a standardized Percoll gradient proved to be highly effective for the separation/enrichment of these cells, without damaging the cell membrane. Pokeweed and phytohemagglutinin showed a high stimulating effect, with proliferation rates of 16,753 and 2350 counts/min (cpm), respectively, whereas Con A showed very low stimulation indices (495 cpm), supporting the notion that it should not be used for blast proliferation studies in goats. |
Palabras clave : | PWM Con A PHA Proliferation Goats |
URI : | http://www.repositorio.ufba.br/ri/handle/ri/7459 |
Fecha de publicación : | 2002 |
Aparece en las colecciones: | Artigo Publicado em Periódico Nacional (ISC) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
1-s2.0-S0921448802001542-main.pdf Restricted Access | 65,45 kB | Adobe PDF | Visualizar/Abrir Request a copy |
Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.