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Please use this identifier to cite or link to this item: http://repositorio.ufba.br/ri/handle/ri/7459

Title: Effect of different lectins on the proliferation of goat peripheral blood mononuclear cells
Other Titles: Small Ruminant Research
Authors: Regis, Lia Fernandes
Ribeiro, Marcos
Vale, Vera
Paule, Bruno
Nascimento, Ivana Lúcia de Oliveira
Schaer, Robert Eduard
Nascimento, Roberto José Meyer
Freire, Songeli
Keywords: PWM;Con A;PHA;Proliferation;Goats
Issue Date: 2002
Abstract: In the present study we determined the effect of the lectins concanavalin A (Con A), phytohemagglutinin (PHA) and pokeweed (PWM), frequently used in cell proliferation assays, on the proliferation of goat peripheral blood mononuclear cells (PBMCs). The use of a standardized Percoll gradient proved to be highly effective for the separation/enrichment of these cells, without damaging the cell membrane. Pokeweed and phytohemagglutinin showed a high stimulating effect, with proliferation rates of 16,753 and 2350 counts/min (cpm), respectively, whereas Con A showed very low stimulation indices (495 cpm), supporting the notion that it should not be used for blast proliferation studies in goats.
Description: Texto completo: acesso restrito. p. 71-73
URI: http://www.repositorio.ufba.br/ri/handle/ri/7459
ISSN: 0921-4488
Appears in Collections:Artigos Publicados em Periódicos Nacionais (ISC)

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