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metadata.dc.type: | Artigo de Periódico |
Title: | Characterization of the immune response to Leishmania intum recombinant antigensfan |
Other Titles: | Microbes and Infection |
Authors: | Carvalho, Lucas Pedreira de Soto, Manuel Jerônimo, Selma Dondji, Blaise Bacellar, Maria Olívia Amado Ramos Luz, Viviane Orge, Glória Orge Alonso, Carlos Jesus, Amélia R. Carvalho Filho, Edgar Marcelino de |
metadata.dc.creator: | Carvalho, Lucas Pedreira de Soto, Manuel Jerônimo, Selma Dondji, Blaise Bacellar, Maria Olívia Amado Ramos Luz, Viviane Orge, Glória Orge Alonso, Carlos Jesus, Amélia R. Carvalho Filho, Edgar Marcelino de |
Abstract: | Leishmaniases have a high prevalence in tropical countries. In order to improve existing diagnostic systems based on total Leishmania proteins, and to identify antigen candidates for vaccine development, an intensive search for the identification of antigens was performed using molecular biology techniques. In this study, the immune response to three L. infantum recombinant antigens was evaluated. Upon stimulation with KMP11, mononuclear cells from leishmaniasis patients produced high levels of IL-10, while a predominant IFN-γ production could be observed in cultures stimulated with H2A and soluble Leishmania antigen. All the recombinant antigens induced very little IL-5. KMP11 decreased IFN-γ production by 48% in cultures of peripheral blood mononuclear cells from cutaneous leishmaniasis patients who had been stimulated with soluble Leishmania antigen. Furthermore, antibodies to KMP11 were detected in the sera from all patients with visceral leishmaniasis and in the majority of the sera from patients with cutaneous leishmaniasis or individuals with asymptomatic L. chagasi infection. Thus, KMP11 is recognized by cells and sera of patients with different clinical forms of leishmaniasis, and KMP11, through IL-10 production, proved to be a potent antigen in modulating type 1 immune response. |
Keywords: | Leishmaniasis Immuneregulation IFN-γ KMP11 |
URI: | http://www.repositorio.ufba.br/ri/handle/ri/7374 |
Issue Date: | 2003 |
Appears in Collections: | Artigo Publicado em Periódico (ICS) |
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1-s2.0-S1286457902000515-main.pdf Restricted Access | 112,05 kB | Adobe PDF | View/Open Request a copy |
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