Please use this identifier to cite or link to this item: https://repositorio.ufba.br/handle/ri/5857
metadata.dc.type: Artigo de Periódico
Title: Invasion of Ureaplasma diversum in Hep-2 cells
Other Titles: BMC Microbiology
Authors: Marques, Lucas Miranda
Ueno, Priscilla Megumi
Buzinhani, Melissa
Cortez, Beatriz de Araujo
L. Neto, Renata
Yamaguti, Maurício
Oliveira, Rosângela C.
Guimarães, Ana Márcia S.
Monezi, Telma Alves
Braga Júnior, Antônio Carlos Ricardo
metadata.dc.creator: Marques, Lucas Miranda
Ueno, Priscilla Megumi
Buzinhani, Melissa
Cortez, Beatriz de Araujo
L. Neto, Renata
Yamaguti, Maurício
Oliveira, Rosângela C.
Guimarães, Ana Márcia S.
Monezi, Telma Alves
Braga Júnior, Antônio Carlos Ricardo
Abstract: Background: Understanding mollicutes is challenging due to their variety and relationship with host cells. Invasion has explained issues related to their opportunistic role. Few studies have been done on the Ureaplasma diversum mollicute, which is detected in healthy or diseased bovine. The invasion in Hep-2 cells of four clinical isolates and two reference strains of their ureaplasma was studied by Confocal Laser Scanning Microscopy and gentamicin invasion assay. Results: The isolates and strains used were detected inside the cells after infection of one minute without difference in the arrangement for adhesion and invasion. The adhesion was scattered throughout the cells, and after three hours, the invasion of the ureaplasmas surrounded the nuclear region but were not observed inside the nuclei. The gentamicin invasion assay detected that 1% of the ATCC strains were inside the infected Hep-2 cells in contrast to 10% to the clinical isolates. A high level of phospholipase C activity was also detected in all studied ureaplasma. Conclusions: The results presented herein will help better understand U. diversum infections, aswell as cellular attachment and virulence.
Publisher: BioMed
URI: http://www.repositorio.ufba.br/ri/handle/ri/5857
Issue Date: 2010
Appears in Collections:Artigo Publicado em Periódico (IMS)

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