Please use this identifier to cite or link to this item: https://repositorio.ufba.br/handle/ri/5450
metadata.dc.type: Artigo de Periódico
Title: Intraspecific sequence variation in 16S rRNA gene of Ureaplasma diversum isolates
Other Titles: Veterinary Microbiology
Authors: Timenetsky, Jorge
Marques, Lucas Miranda
Buzinhani, Melissa
Guimarães, Ana Marcia de Sá
Oliveira, Rosângela Claret de
Yamaguti, Maurício
Marques, R. C. P.
Farias, S. T.
Lopes Neto, R.
metadata.dc.creator: Timenetsky, Jorge
Marques, Lucas Miranda
Buzinhani, Melissa
Guimarães, Ana Marcia de Sá
Oliveira, Rosângela Claret de
Yamaguti, Maurício
Marques, R. C. P.
Farias, S. T.
Lopes Neto, R.
Abstract: Ureaplasma diversum infection in bulls may result in seminal vesiculitis, balanoposthitis and alterations in spermatozoids. In cows, it can cause placentitis, fetal alveolitis, abortion and the birth of weak calves. U. diversum ATCC 49782 (serogroups A), ATCC 49783 (serogroup C) and 34 field isolates were used for this study. These microorganisms were submitted to Polymerase Chain Reaction for 16S gene sequence determination using Taq High Fidelity and the products were purified and bi-directionally sequenced. Using the sequence obtained, a fragment containing four hypervariable regions was selected and nucleotide polymorphisms were identified based on their position within the 16S rRNA gene. Forty-four single nucleotide polymorphisms (SNP) were detected. The genotypic variability of the 16S rRNA gene of U. diversum isolates shows that the taxonomy classification of these organisms is likely much more complex than previously described and that 16S rRNA gene sequencing may be used to suggest an epidemiologic pattern of different origin strains.
Keywords: Ureaplasma diversum
Intraspecific variation
16S rRNA gene
URI: http://www.repositorio.ufba.br/ri/handle/ri/5450
Issue Date: 2011
Appears in Collections:Artigo Publicado em Periódico (IMS)

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