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Please use this identifier to cite or link to this item: http://repositorio.ufba.br/ri/handle/ri/5422

Title: TNF-alpha and IL-8: Serum levels and gene polymorphisms (ÿ308G>A and ÿ251A>T) are associated with classical biomarkers and medical history in children with sickle cell anemia
Other Titles: Cytokine
Authors: Lyra, Isa Menezes
Moura Neto, José Pereira de
Reis, Mitermayer Galvão dos
Barbosa, Cynara Gomes
Gonçalves, Marilda de Souza
Cerqueira, Bruno Antonio Veloso
Couto, Fábio David
Cajado, C.
Santos, Wendell Vilas Boas
Dorea, M. J.
Keywords: TNF-alpha;IL-8;Sickle cell anemia;betaS-globin Gene haplotypes;alpha2-Thalassemia
Issue Date: 2011
Abstract: Sickle cell anemia (SCA) is a disorder characterized by a heterogeneous clinical outcome. In the present study, we investigated the associations between Tumor Necrosis Factor-alpha (TNF-alpha) ÿ308G>A and Interleukin 8 (IL-8) ÿ251A>T gene polymorphisms, medical history and classical biomarkers in children with steady-state SCA. In total, 210 SCA patients aged 2–21 years and 200 healthy controls were studied. Gene polymorphisms, betaS-globin haplotypes and a 3.7-kb deletion in alpha2-thalassemia (a2-thal3.7 kb) were investigated by PCR/RFLP analysis, and cytokine levels were determined by ELISA. Splenomegaly (p = .032) was more prevalent among children younger than 5 years of age. The A allele of the TNF-alpha ÿ308G>A gene polymorphism and the presence of a2-thal3.7 kb were associated with an increase risk of splenic sequestration events (p = .001; p = .046), while the T allele of the IL-8 ÿ251A>T gene polymor-phism was considered to be a protective factor for splenomegaly events (p = .032). Moreover, the A allele of the TNF-alpha ÿ308G>A gene polymorphism was associated with high TNF-alpha levels (p = .021), and the hemoglobin F and hemoglobin S haplotypes were correlated with serum levels of IL-8. The logistic regression analysis showed significant effects of the TNF-alpha and IL-8 gene polymorphisms, betaS-globin gene haplotypes and a2-thal3.7 kb on the occurrence of splenic sequestration events. Our study empha-sizes that the identification of new genetic and immunological biomarkers and their associations with classical markers is an important strategy to elucidate the underlying causes of different SCA phenotypes and their effects on patient outcome.
Description: texto completo: acesso restrito. p. 312–317.
URI: http://www.repositorio.ufba.br/ri/handle/ri/5422
ISSN: 1096-0023
Appears in Collections:Artigos Publicados em Periódicos (FARMACIA)

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