Skip navigation
Universidade Federal da Bahia |
Repositório Institucional da UFBA
Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/4804
Tipo: Artigo de Periódico
Título: Production of Monoclonal Antibodies Against Canine Leukocytes
Título(s) alternativo(s): Hybridoma And Hybridomics
Autor(es): Aguiar, Paulo Henrique Palis
Santos, Roberto Robson Borges dos
Lima, Carla Andrade
Gomes, Hilton Rios de Sousa
Larangeira, Daniela Farias
Santos, Patrícia Meira
Melo, Stella Maria Barrouin
Santos, Washington Luis Conrado dos
Carvalho, Lain Carlos Pontes de
Autor(es): Aguiar, Paulo Henrique Palis
Santos, Roberto Robson Borges dos
Lima, Carla Andrade
Gomes, Hilton Rios de Sousa
Larangeira, Daniela Farias
Santos, Patrícia Meira
Melo, Stella Maria Barrouin
Santos, Washington Luis Conrado dos
Carvalho, Lain Carlos Pontes de
Abstract: A panel of anti-canine leukocyte monoclonal antibodies (MAbs) was produced by immunizing BALB/c mice with canine peripheral blood mononuclear cells (PBMC), either resting or stimulated with concanavalin A (ConA). Three out of 28 clones—IH1, AB6, and HG6–screened by ELISA and producing antibody with the highest specificity for canine cell immunostaining, were subjected to three subsequent subcloning steps by limiting dilution, and selected for further characterization. These MAbs belonged to IgG1 (HG6 and IH1) and IgG2a (AB6) isotypes. The distribution of cell populations expressing the antigen recognized by the antibodies was identified by indirect immunoflorescence on canine PBMC and on tissue sections of lymph node, spleen, liver and skin. The possible crossreactivity with human PBMC was also examined in immunocytochemistry. One of the antibodies specifically recognized macrophages. The MAbs presented here can be foreseen as possible valuable diagnostic and research tools to study immune functions in dogs.
URI: http://www.repositorio.ufba.br/ri/handle/ri/4804
Data do documento: 2004
Aparece nas coleções:Artigo Publicado em Periódico (EMV)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
153685904774129739.pdf350,91 kBAdobe PDFVisualizar/Abrir
Mostrar registro completo do item Visualizar estatísticas


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.