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dc.contributor.authorAguiar, Paulo Henrique Palis-
dc.contributor.authorSantos, Roberto Robson Borges dos-
dc.contributor.authorLima, Carla Andrade-
dc.contributor.authorGomes, Hilton Rios de Sousa-
dc.contributor.authorLarangeira, Daniela Farias-
dc.contributor.authorSantos, Patrícia Meira-
dc.contributor.authorMelo, Stella Maria Barrouin-
dc.contributor.authorSantos, Washington Luis Conrado dos-
dc.contributor.authorCarvalho, Lain Carlos Pontes de-
dc.creatorAguiar, Paulo Henrique Palis-
dc.creatorSantos, Roberto Robson Borges dos-
dc.creatorLima, Carla Andrade-
dc.creatorGomes, Hilton Rios de Sousa-
dc.creatorLarangeira, Daniela Farias-
dc.creatorSantos, Patrícia Meira-
dc.creatorMelo, Stella Maria Barrouin-
dc.creatorSantos, Washington Luis Conrado dos-
dc.creatorCarvalho, Lain Carlos Pontes de-
dc.descriptionp. 127-132pt_BR
dc.description.abstractA panel of anti-canine leukocyte monoclonal antibodies (MAbs) was produced by immunizing BALB/c mice with canine peripheral blood mononuclear cells (PBMC), either resting or stimulated with concanavalin A (ConA). Three out of 28 clones—IH1, AB6, and HG6–screened by ELISA and producing antibody with the highest specificity for canine cell immunostaining, were subjected to three subsequent subcloning steps by limiting dilution, and selected for further characterization. These MAbs belonged to IgG1 (HG6 and IH1) and IgG2a (AB6) isotypes. The distribution of cell populations expressing the antigen recognized by the antibodies was identified by indirect immunoflorescence on canine PBMC and on tissue sections of lymph node, spleen, liver and skin. The possible crossreactivity with human PBMC was also examined in immunocytochemistry. One of the antibodies specifically recognized macrophages. The MAbs presented here can be foreseen as possible valuable diagnostic and research tools to study immune functions in dogs.pt_BR
dc.titleProduction of Monoclonal Antibodies Against Canine Leukocytespt_BR
dc.title.alternativeHybridoma And Hybridomicspt_BR
dc.typeArtigo de Periódicopt_BR
dc.identifier.numberv. 23, n.2.pt_BR
Appears in Collections:Artigo Publicado em Periódico (EMV)

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