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Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/20345
Tipo: Artigo de Periódico
Título: SDS-PAGE and Western blot analysis of somatic and extracellular antigens of Corynebacterium pseudotuberculosis
Título(s) alternativo(s): Revista de Ciências Médicas e Biológicas
Autor(es): Paule, Bruno Jean Adrien
Azevedo, Vasco Ariston de Carvalho
Costa, Lilia Ferreira Moura
Freire, Songeli Menezes
Regis, Lia Fernandes
Vale, Vera Lúcia Costa
Bahia, Robson Cerqueira
Carminati, Renato
Nascimento, Ivana Lúcia de Oliveira
Nascimento, Roberto José Meyer
Autor(es): Paule, Bruno Jean Adrien
Azevedo, Vasco Ariston de Carvalho
Costa, Lilia Ferreira Moura
Freire, Songeli Menezes
Regis, Lia Fernandes
Vale, Vera Lúcia Costa
Bahia, Robson Cerqueira
Carminati, Renato
Nascimento, Ivana Lúcia de Oliveira
Nascimento, Roberto José Meyer
Abstract: Different techniques to isolate secreted, cell-surface located and somatic fractions of Corynebacterium pseudotuberculosis have been studied by SDS-PAGE and immunoblotting, by using a pool of sera from naturally infected goats. The secreted antigens were obtained from culture supernatant of the bacteria grown in a chemically defined medium or in Brain Heart Infusion medium. The surface fraction was obtained by NaCl 1 M treatment and the somatic ones were obtained by using distinct procedures (detergents and ultrasounds). By Coomassie blue staining, we observed 20 bands in the secreted fraction, 35 bands in the surface extract and 40 to 50 bands in the somatic fraction, depending on the extraction procedure. Sixteen immunoreactive proteins were detected among the studied fractions. Bands with molecular weights of 125, 108, 75, 68, 41, 40, 31 and 24 kDa were recognized with higher intensity and all of them were found in the secreted fraction. The use of the chemically defined medium allowed us to evidence the presence of proteins with high molecular masses in the secreted fraction of C. pseudotuberculosis, which had not been previously described.
Palavras-chave: Corynebacterium pseudotuberculosis.
Goat.
Antigens.
SDS-PAGE.
Western blot.
Editora / Evento / Instituição: Instituto de Ciências da Saúde/ Universidade Federal da Bahia
Citação: PAULE, B. J. A. et aL. SDS-PAGE and Western blot analysis of somatic and extracellular antigens of Corynebacterium pseudotuberculosis. R. Ci. méd. biol., Salvador, v. 3, n. 1, p. 44-52, jan./jun. 2004.
Tipo de Acesso: Acesso Aberto
URI: http://repositorio.ufba.br/ri/handle/ri/20345
Data do documento: Jan-2004
Aparece nas coleções:Artigo Publicado em Periódico (PPGPIOS)

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