Campo DC | Valor | Idioma |
dc.contributor.author | Paule, Bruno Jean Adrien | - |
dc.contributor.author | Azevedo, Vasco Ariston de Carvalho | - |
dc.contributor.author | Costa, Lilia Ferreira Moura | - |
dc.contributor.author | Freire, Songeli Menezes | - |
dc.contributor.author | Regis, Lia Fernandes | - |
dc.contributor.author | Vale, Vera Lúcia Costa | - |
dc.contributor.author | Bahia, Robson Cerqueira | - |
dc.contributor.author | Carminati, Renato | - |
dc.contributor.author | Nascimento, Ivana Lúcia de Oliveira | - |
dc.contributor.author | Nascimento, Roberto José Meyer | - |
dc.creator | Paule, Bruno Jean Adrien | - |
dc.creator | Azevedo, Vasco Ariston de Carvalho | - |
dc.creator | Costa, Lilia Ferreira Moura | - |
dc.creator | Freire, Songeli Menezes | - |
dc.creator | Regis, Lia Fernandes | - |
dc.creator | Vale, Vera Lúcia Costa | - |
dc.creator | Bahia, Robson Cerqueira | - |
dc.creator | Carminati, Renato | - |
dc.creator | Nascimento, Ivana Lúcia de Oliveira | - |
dc.creator | Nascimento, Roberto José Meyer | - |
dc.date.accessioned | 2016-09-13T15:16:00Z | - |
dc.date.available | 2016-09-13T15:16:00Z | - |
dc.date.issued | 2004-01 | - |
dc.identifier.citation | PAULE, B. J. A. et aL. SDS-PAGE and Western blot analysis of somatic and extracellular antigens of Corynebacterium pseudotuberculosis. R. Ci. méd. biol., Salvador, v. 3, n. 1, p. 44-52, jan./jun. 2004. | pt_BR |
dc.identifier.issn | 2236-5222 | - |
dc.identifier.uri | http://repositorio.ufba.br/ri/handle/ri/20345 | - |
dc.description | Artigo original(p.44-52) | pt_BR |
dc.description.abstract | Different techniques to isolate secreted, cell-surface located and somatic fractions of Corynebacterium pseudotuberculosis
have been studied by SDS-PAGE and immunoblotting, by using a pool of sera from naturally infected goats. The
secreted antigens were obtained from culture supernatant of the bacteria grown in a chemically defined medium or in
Brain Heart Infusion medium. The surface fraction was obtained by NaCl 1 M treatment and the somatic ones were
obtained by using distinct procedures (detergents and ultrasounds). By Coomassie blue staining, we observed 20
bands in the secreted fraction, 35 bands in the surface extract and 40 to 50 bands in the somatic fraction, depending
on the extraction procedure. Sixteen immunoreactive proteins were detected among the studied fractions. Bands with
molecular weights of 125, 108, 75, 68, 41, 40, 31 and 24 kDa were recognized with higher intensity and all of them
were found in the secreted fraction. The use of the chemically defined medium allowed us to evidence the presence of
proteins with high molecular masses in the secreted fraction of C. pseudotuberculosis, which had not been previously
described. | pt_BR |
dc.language.iso | pt_BR | pt_BR |
dc.publisher | Instituto de Ciências da Saúde/ Universidade Federal da Bahia | pt_BR |
dc.rights | Acesso Aberto | pt_BR |
dc.source | http://www.portalseer.ufba.br/index.php/cmbio/issue/view/498/showToc | pt_BR |
dc.subject | Corynebacterium pseudotuberculosis. | pt_BR |
dc.subject | Goat. | pt_BR |
dc.subject | Antigens. | pt_BR |
dc.subject | SDS-PAGE. | pt_BR |
dc.subject | Western blot. | pt_BR |
dc.title | SDS-PAGE and Western blot analysis of somatic and extracellular antigens of Corynebacterium pseudotuberculosis | pt_BR |
dc.title.alternative | Revista de Ciências Médicas e Biológicas | pt_BR |
dc.type | Artigo de Periódico | pt_BR |
dc.description.localpub | Salvador | pt_BR |
dc.identifier.number | v.3, n.1 | pt_BR |
Aparece nas coleções: | Artigo Publicado em Periódico (PPGPIOS)
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