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https://repositorio.ufba.br/handle/ri/15887
metadata.dc.type: | Artigo de Periódico |
Title: | PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania |
Other Titles: | Nanomedicine: Nanotechnology, Biology and Medicine |
Authors: | Santos, Diego M. Carneiro, Marcia W. Moura, Tatiana Rodrigues de Soto, Manuel Luz, Nívea Farias Prates, Deboraci Brito Barral, Aldina Maria Prado |
metadata.dc.creator: | Santos, Diego M. Carneiro, Marcia W. Moura, Tatiana Rodrigues de Soto, Manuel Luz, Nívea Farias Prates, Deboraci Brito Barral, Aldina Maria Prado |
Abstract: | We recently demonstrated that immunization with polyester poly(lactide-co-glycolide acid) (PLGA) nanoparticles loaded with the 11-kDa Leishmania vaccine candidate kinetoplastid membrane protein 11 (KMP-11) significantly reduced parasite load in vivo. Presently, we explored the ability of the recombinant PLGA nanoparticles to stimulate innate responses in macrophages and the outcome of infection with Leishmania braziliensis in vitro. Incubation of macrophages with KMP-11-loaded PLGA nanoparticles significantly decreased parasite load. In parallel, we observed the augmented production of nitric oxide, superoxide, TNF-α and IL-6. An increased release of CCL2/MCP-1 and CXCL1/KC was also observed, resulting in macrophage and neutrophil recruitment in vitro. Lastly, the incubation of macrophages with KMP-11-loaded PLGA nanoparticles triggered the activation of caspase-1 and the secretion of IL-1β and IL-18, suggesting inflammasome participation. Inhibition of caspase-1 significantly increased the parasite load. We conclude that KMP-11-loaded PLGA nanoparticles promote the killing of intracellular Leishmania parasites through the induction of potent innate responses. |
Keywords: | Leishmania PLGA Nanoparticle Macrophage Innate response |
metadata.dc.rights: | Acesso Aberto |
URI: | http://repositorio.ufba.br/ri/handle/ri/15887 |
Issue Date: | 2013 |
Appears in Collections: | Artigo Publicado em Periódico (FAR) |
Files in This Item:
File | Description | Size | Format | |
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1-s2.0-S1549963413001755-main.pdf | 1,35 MB | Adobe PDF | View/Open |
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