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metadata.dc.type: | Artigo de Periódico |
Title: | Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil |
Other Titles: | Mem Inst Oswaldo Cruz |
Authors: | Bueris, Vanessa Sircili, Marcelo Palma Taddei, Carla Romano Santos, Maurilio Fernandes dos Franzolin, Marcia Regina Martinez, Marina Baquerizo Ferrer, Suzana Ramos Barreto, Mauricio Lima Trabulsi, Luiz Rachid |
metadata.dc.creator: | Bueris, Vanessa Sircili, Marcelo Palma Taddei, Carla Romano Santos, Maurilio Fernandes dos Franzolin, Marcia Regina Martinez, Marina Baquerizo Ferrer, Suzana Ramos Barreto, Mauricio Lima Trabulsi, Luiz Rachid |
Abstract: | We identified different diarrheagenic (DEC) Escherichia coli pathotypes isolated from 1,207 children with and without acute endemic diarrhea in Salvador, Bahia, Brazil collected as part of a case control study. Since the identification of DEC cannot be based on only biochemical and culture criteria, we used a multiplex polymerase chain reaction developed by combining five specific primer pairs for Enteropathogenic Escherichia coli (EPEC), Shiga toxin-producing E. coli/ Enterohaemorrhagic E. coli (STEC/EHEC), Enterotoxigenic E. coli (ETEC) and Enteroaggregative E. coli (EAEC) to detect these pathotypes simultaneously in a single-step reaction. In order to distinguish typical and atypical EPEC strains, these were tested for the presence of EAF plasmid. The prevalence of diarrheagenic E. coli in this sample of a global case-control study was 25.4% (259 patients) and 18.7% (35 patients) in the diarrhea group (1,020 patients) and the control group (187 patients), respectively. The most frequently isolated pathotype was EAEC (10.7%), followed by atypical EPEC (9.4%), ETEC (3.7%), and STEC (0.6%). Typical EPEC was detected only in one sample. The prevalence of the pathotypes studied in children with diarrhea was not significantly different from that in children without diarrhea. |
Keywords: | diarrheagenic Escherichia coli virulence Multiplex PCR |
URI: | http://www.repositorio.ufba.br/ri/handle/ri/1551 |
Issue Date: | 2007 |
Appears in Collections: | Artigo Publicado em Periódico Nacional (ISC) |
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arrtigo repositorio16.pdf | 164,18 kB | Adobe PDF | View/Open |
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