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metadata.dc.type: | Artigo de Periódico |
Title: | Agathisflavone Enhances Retinoic Acid-Induced Neurogenesis and Its Receptors α and β in Pluripotent Stem Cells |
Other Titles: | Stem Cells and Development |
Authors: | Souza, Cleide dos Santos Chicaybam, Leonardo Bonamino, Martin Hernán Bahia, Marcus Vinicius Costa, Silvia Lima Borges, Helena L. Rehen, Stevens K. |
metadata.dc.creator: | Souza, Cleide dos Santos Chicaybam, Leonardo Bonamino, Martin Hernán Bahia, Marcus Vinicius Costa, Silvia Lima Borges, Helena L. Rehen, Stevens K. |
Abstract: | Flavonoids have key functions in the regulation of multiple cellular processes; however, their effects have been poorly examined in pluripotent stem cells. Here, we tested the hypothesis that neurogenesis induced by all-trans retinoic acid (RA) is enhanced by agathisflavone (FAB, Caesalpinia pyramidalis Tull). Mouse embryonic stem (mES) cells and induced pluripotent stem (miPS) cells growing as embryoid bodies (EBs) for 4 days were treated with FAB (60 μM) and/or RA (2 μM) for additional 4 days. FAB did not interfere with the EB mitotic rate of mES cells, as evidenced by similar percentages of mitotic figures labeled by phospho-histone H3 in control (3.4%±0.4%) and FAB-treated groups (3.5%±1.1%). Nevertheless, the biflavonoid reduced cell death in both control and RA-treated EBs from mES cells by almost 2-fold compared with untreated EBs. FAB was unable, by itself, to induce neuronal differentiation in EBs after 4 days of treatment. On the other hand, FAB enhanced neuronal differentiation induced by RA in both EBs of mES and miPS. FAB increased the percentage of nestin-labeled cells by 2.7-fold (mES) and 2.4 (miPS) and β-tubulin III–positive cells by 2-fold (mES) and 2.7 (miPS) in comparison to RA-treated EBs only. FAB increased the expression of RA receptors α and β in mES EBs, suggesting that the availability of RA receptors is limiting RA-induced neurogenesis in pluripotent stem cells. This is the first report to describe that naturally occurring biflavonoids regulate apoptosis and neuronal differentiation in pluripotent stem cells. |
metadata.dc.rights: | Acesso Aberto |
URI: | http://repositorio.ufba.br/ri/handle/ri/14750 |
Issue Date: | 2011 |
Appears in Collections: | Artigo Publicado em Periódico (ICS) |
Files in This Item:
File | Description | Size | Format | |
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Cleide S. Souza.pdf | 782,54 kB | Adobe PDF | View/Open |
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