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Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/11969
Tipo: Artigo de Periódico
Título: The role of glycosylated epitopes in the serodiagnosis of Strongyloides stercoralis infection
Título(s) alternativo(s): Diagnostic Microbiology and Infectious Disease
Autor(es): Inês, Elizabete de Jesus
Silva, Mônica Lopes Sampaio
Souza, Joelma Nascimento
Teixeira, Márcia Cristina Aquino
Soares, Neci Matos
Autor(es): Inês, Elizabete de Jesus
Silva, Mônica Lopes Sampaio
Souza, Joelma Nascimento
Teixeira, Márcia Cristina Aquino
Soares, Neci Matos
Abstract: Carbohydrates of pathogen antigens have been disrupted by periodate oxidation, in order to reduce nonspecific bindings and improve serodiagnosis of parasite infections. In the present study, the enzymelinked immunosorbent assay (ELISA) was carried out with filariform larvae antigen treated, or not treated, with sodium metaperiodate. Groups of sera from patients with Strongyloides stercoralis infection, with other intestinal parasites and a normal control, were used. The oxidation of Strongyloides stercoralis glycosylated epitopes reduced the seroreactivity of sera from patients with S. stercoralis infection as demonstrated by ELISA, with a decrease in sera optical densities. The number of cross-reactions of IgG and IgE-ELISAs increased by 12% and 16%, respectively, after antigen treatment with metaperiodate. This was more often observed in patients infected with Schistosoma mansoni and hookworm. Moreover, the IgG depletion from sera tested by IgE-ELISA led to the detection of previous false-negative samples from S. stercoralis–infected patients.
Palavras-chave: Strongyloides stercoralis
Sodium metaperiodate
Serodiagnosis
Glycoproteins
Editora / Evento / Instituição: Diagnostic Microbiology and Infectious Disease
URI: http://www.repositorio.ufba.br/ri/handle/ri/11969
Data do documento: 2013
Aparece nas coleções:Artigo Publicado em Periódico (FAR)

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