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https://repositorio.ufba.br/handle/ri/8317
metadata.dc.type: | Artigo de Periódico |
Title: | The use of metal chelate affinity chromatography on the isolation of Leishmania chagasi promastigote hydrophobic proteinases |
Other Titles: | Veterinary Parasitology |
Authors: | Alves, J. L. Lima, F. W. Mendonça Alves, C. R. |
metadata.dc.creator: | Alves, J. L. Lima, F. W. Mendonça Alves, C. R. |
Abstract: | In this work, we have assessed the possibility of isolating metalloproteinase fractions from infective Leishmania chagasi promastigotes. Our strategy was the association of the Triton X-114 method with iminodiacetic chromatography enriched with Zn2+. Thus, by using acid conditions, it was possible to isolate two fractions containing two polypeptides, 59 and 63 kDa. The enzymatic activity assay indicated that the two fractions and the two polypeptides had proteinase activities. In addition, it was proposed that those proteinase activities were affected by the presence of 1,10-phenanthroline, a metalloproteinase inhibitor. With this gentle chromatography strategy proposed it is possible to obtain metalloproteinases from L. chagasi in folding that preserve the enzyme activity. This is important for further studies on pathological complications observed in visceral leishmaniasis. |
Keywords: | Leishmania chagasi Chromatography Metalloproteinase |
Publisher: | Elsevier |
URI: | http://www.repositorio.ufba.br/ri/handle/ri/8317 |
Issue Date: | 2003 |
Appears in Collections: | Artigo Publicado em Periódico (Biologia) |
Files in This Item:
File | Description | Size | Format | |
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1-s2.0-S0304401703004631-main.pdf Restricted Access | 120,15 kB | Adobe PDF | View/Open Request a copy |
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