Please use this identifier to cite or link to this item:
https://repositorio.ufba.br/handle/ri/8286
metadata.dc.type: | Artigo de Periódico |
Title: | Activating mutation of the stimulatory G protein (gsp) as a putative cause of ovarian and testicular human stromal Leydig cell tumors |
Other Titles: | Journal of Clinical Endocrinology and Metabolism |
Authors: | Fragoso, Maria Candida B. Villares Latronico, Ana Claudia Carvalho, Filomena Marino Zerbini, Maria Claudia N. Marcondes, Jose Antonio Miguel Araujo, Leila M. B. Lando, Valeria S. Frazzatto, Eliana T. Mendonca, Berenice B. Villares, Sandra Mara F. |
metadata.dc.creator: | Fragoso, Maria Candida B. Villares Latronico, Ana Claudia Carvalho, Filomena Marino Zerbini, Maria Claudia N. Marcondes, Jose Antonio Miguel Araujo, Leila M. B. Lando, Valeria S. Frazzatto, Eliana T. Mendonca, Berenice B. Villares, Sandra Mara F. |
Abstract: | Activating mutations of the G protein genes have been associated with the development of several endocrine neoplasms. Such activating mutations, gip2, affecting the α-subunit of the Gαi2 protein were previously described by a single group in 30% of ovarian sex cord stromal tumors. Other activating mutations of the α-subunit of the Gs (gsp) have been identified in GH-secreting and nonfunctioning pituitary tumors, autonomous thyroid adenomas, and all affected McCune-Albright tissues, but not in sex cord stromal tumors. In the present study, we investigated the presence of gip2 and gsp mutations in 14 human sex cord stromal tumors. Six Leydig cell tumors (4 ovaries and 2 testes), 2 thecomas, 2 granulosa cell tumors, 3 androblastomas, and 1 gonadoblastoma (sex cord and germ cell) were included in this study. Genomic DNA was obtained from either fresh-frozen tumor tissues or paraffin-embedded sections and in some cases from blood samples. Using PCR, denaturing gradient gel electrophoresis, and direct sequencing, we detected 4 tumors (66.6%) with the gsp mutation (R201C) in our series of ovarian and testicular Leydig cell tumors. In contrast, no gip2 mutations were found in any of the sex cord stromal tumors studied. In conclusion, our findings suggest that the putative oncogene gsp may play a significant role in the molecular mechanism of these tumors. |
URI: | http://www.repositorio.ufba.br/ri/handle/ri/8286 |
Issue Date: | 1998 |
Appears in Collections: | Artigo Publicado em Periódico (Faculdade de Medicina) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Fragoso.pdf Restricted Access | 959,69 kB | Adobe PDF | View/Open Request a copy |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.