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Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/7437
Tipo: Artigo de Periódico
Título: Up-regulation of th1-type responses in mucosal leishmaniasis patients
Título(s) alternativo(s): Infection and Immunity
Autor(es): Bacellar, Maria Olívia Amado Ramos
Lessa, Hélio Andrade
Schriefer, Nicolaus Albert Borges
Machado, Paulo Roberto Lima
Jesus, Amélia Ribeiro de
Dutra, Walderez Ornelas
Gollob, Kenneth John
Carvalho Filho, Edgar Marcelino de
Autor(es): Bacellar, Maria Olívia Amado Ramos
Lessa, Hélio Andrade
Schriefer, Nicolaus Albert Borges
Machado, Paulo Roberto Lima
Jesus, Amélia Ribeiro de
Dutra, Walderez Ornelas
Gollob, Kenneth John
Carvalho Filho, Edgar Marcelino de
Abstract: The cytokine profile produced by peripheral blood mononuclear cells (PBMC) in response to leishmania antigens and the ability of interleukin-10 (IL-10) and transforming growth factor β (TGF-β) to modulate the immune response were evaluated in 21 mucosal leishmaniasis patients. Patients with mucosal disease exhibited increased gamma interferon (IFN-γ) and tumor necrosis factor alpha (TNF-α) secretion and decreased IL-10 secretion compared to patients with classical cutaneous leishmaniasis. CD4+ Th1 cells were the main source of IFN-γ and TNF-α production in mucosal leishmaniasis patients. Evaluation of cytokine gene expression in PBMC of these patients showed that there was strong up-regulation of IFN-γ transcripts upon stimulation with leishmania antigen, in contrast to the baseline levels of IL-10 mRNA. IL-10 suppressed IFN-γ production by 48% in cell cultures from cutaneous leishmaniasis patients and by 86% in cell cultures from healthy subjects stimulated with purified protein derivative, whereas in similar conditions IL-10 suppressed IFN-γ production by 19% in cell cultures from mucosal leishmaniasis patients stimulated with leishmania antigen. TGF-β suppressed IFN-γ levels to a greater extent in healthy subjects than in mucosal leishmaniasis and cutaneous leishmaniasis patients. These data indicate that a poorly modulated T-cell response in mucosal leishmaniasis patients leads to production of high levels of proinflammatory cytokines, such as IFN-γ and TNF-α, as well as a decreased ability of IL-10 and TGF-β to modulate this response. These abnormalities may be the basis for the pathological findings observed in this disease.
URI: http://www.repositorio.ufba.br/ri/handle/ri/7437
Data do documento: 2002
Aparece nas coleções:Artigo Publicado em Periódico (PPGCS)

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