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metadata.dc.type: Artigo de Periódico
Título : Role of costimulatory molecules in immune response of patients with cutaneous leishmaniasis
Otros títulos : Microbes and Infection
Autor : Favali, Cecilia Beatriz Fiuza
Costa, Dirceu Joaquim
Macêdo, Lilian Maria Afonso de
Conceição, Viviane
Rosato, Andréa Bomura
Oliveira, Luiz Fabiano Borges
Costa, Jackson Mauricio Lopes
Barral, Aldina Maria Prado
Barral-Netto, Manoel
Brodskyn, Claudia Ida
metadata.dc.creator: Favali, Cecilia Beatriz Fiuza
Costa, Dirceu Joaquim
Macêdo, Lilian Maria Afonso de
Conceição, Viviane
Rosato, Andréa Bomura
Oliveira, Luiz Fabiano Borges
Costa, Jackson Mauricio Lopes
Barral, Aldina Maria Prado
Barral-Netto, Manoel
Brodskyn, Claudia Ida
Resumen : T cell-mediated immunity is critical in resistance against Leishmania parasites, and T cell activation requires signals provided by costimulatory molecules. Herein we evaluated the role of costimulatory molecules on cytokine production and T cell surface molecule expression by peripheral blood mononuclear cells (PBMC) from cutaneous leishmaniasis (CL) patients. PBMC from CL patients were stimulated with soluble Leishmania antigen (SLA, 10 μg/ml), in the presence or absence of soluble CTLA4-Ig to block CD28-B7 interaction or in the presence or absence of anti-human CD40L to block CD40-CD40L interaction. Supernatants were harvested to evaluate tumor necrosis factor alpha (TNF-α), interleukin 10 (IL-10), transforming growth factor beta (TGF-β) and interferon gamma (IFN-γ) production by ELISA. Cells were harvested after 48 h of culture, stained for specific activation markers and analyzed by flow cytometry. Results show that the blockade of CD28-B7 interaction by CTLA4-Ig downmodulated IFN-γ, IL-10, and TNF-α secretion by PBMC from CL patients. No alteration was detected on either TGF-β production or the expression of CTLA44 or CD25 on CD4+ and CD8+ T cells. When the CD40-CD40L interaction was blockade using anti-CD40L, we did not observe changes in cytokine production or in surface molecule expression. The blockade of the CD28-B7 interactions by CTLA4-Ig also did not alter cytokine production in volunteers immunized against tetanus toxoid (TT). Taken together, these data suggest that the interaction of CTLA4 and CD28-B7 is a TGF-β-independent mechanism that specifically downmodulates the immune response in cutaneous leishmaniasis patients.
Palabras clave : Cutaneous leishmaniasis
Cytokines
Immunomodulation
Editorial : Elsevier
URI : http://www.repositorio.ufba.br/ri/handle/ri/6674
Fecha de publicación : ene-2005
Aparece en las colecciones: Artigo Publicado em Periódico (Faculdade de Medicina)

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