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metadata.dc.type: | Artigo de Periódico |
Title: | Catechol inhibits FADH2-linked respiration in rat liver mitochondrial fraction |
Other Titles: | Acta Cirurgica Brasileira |
Authors: | Barreto, George Emílio Sampaio Santos, Gleide Souza dos Egito, Eryvaldo Sócrates Tabosa El-Bachá, Ramon dos Santos |
metadata.dc.creator: | Barreto, George Emílio Sampaio Santos, Gleide Souza dos Egito, Eryvaldo Sócrates Tabosa El-Bachá, Ramon dos Santos |
Abstract: | PURPOSE: The aim of this work was to investigate the hypothesis that catechol inhibits FADH2-linked basal respiration in mitochondria isolated from rat liver homogenates. Moreover, catechol ability to induce peroxidation of biomolecules in liver nuclear fractions was also studied. METHODS: Rat liver homogenates were incubated with 1mM 1,2-dihydroxybenzene (catechol) at pH 7.4 for up to 30 minutes. After that, mitochondrial fractions were isolated by differential centrifugation. Basal oxygen uptake was measured using a Clark-type electrode after the addition of 10 mM sodium succinate. Nuclear fractions were incubated in the presence of 1 mM catechol for 17 hours at room temperature and the peroxidation of biomolecules was investigated by the reaction with thiobarbituric acid, which was determined spectrophotometrically at 535 nm. RESULTS: Catechol induced a time-dependent partial inhibition of FADH2-linked basal mitochondrial respiration, however this substance was unable to induce a direct peroxidation of biomolecules in hepatic nuclear fractions. CONCLUSION: Catechol produced an inhibition of basal respiration associated to FADH2 in isolated liver mitochondria that could lead to cytotoxicity, ROS generation and cell death. |
Keywords: | Catechol Mitochondrial respiration Peroxidation |
Publisher: | Acta Cirúrgica Brasileira/SOBRADPEC |
URI: | http://www.repositorio.ufba.br/ri/handle/ri/6573 |
Issue Date: | 2005 |
Appears in Collections: | Artigo Publicado em Periódico (Faculdade de Medicina) |
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