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Campo DC | Valor | Idioma |
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dc.contributor.author | Caldwell, Stephen H. | - |
dc.contributor.author | Ikura, Yoshihiro | - |
dc.contributor.author | Dias, Daniela | - |
dc.contributor.author | Isomoto, Kosuke | - |
dc.creator | Caldwell, Stephen H. | - |
dc.creator | Ikura, Yoshihiro | - |
dc.creator | Dias, Daniela | - |
dc.creator | Isomoto, Kosuke | - |
dc.date.accessioned | 2012-04-12T13:39:38Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0168-8278 | - |
dc.identifier.uri | http://www.repositorio.ufba.br/ri/handle/ri/5601 | - |
dc.description | Acesso restrito: Texto completo. p. 719-723. | pt_BR |
dc.description.abstract | Background & Aims: Hepatocellular ballooning is a key finding in nonalcoholic steatohepatitis (NASH). It is conventionally defined by hemotoxylin and eosin (H&E) staining showing enlarged cells with rarefied cytoplasm and recently by changes in the cytoskeleton. Fat droplets are emerging as important organelles in cell metabolism. To address a possible relation between fat droplets and ballooning, we studied fat staining, H&E, and keratin 18 staining in human NASH. Methods: Sequential staining and high resolution imaging were used to study freshly prepared cryo-sections from 10 patients with histologically confirmed steatohepatitis using oil red O for fat droplet identification, H&E to identify ballooning, and anti-K18 to confirm cytoskeletal changes. High resolution images were captured at each stage using the Aperio Scanscope. To provide ultrastructural correlation, glutaraldehyde-fixed specimens were studied using transmission electron microscopy (TEM) with serial sectioning for localization of ballooned cells by light microscopy and TEM in identical specimens. Results: Serial staining consistently demonstrated that hepatocellular ballooning is associated with fat droplet accumulation evident by oil red O positivity and depletion of cytoplasmic keratin 18 with K-18 positive Mallory–Denk bodies (MDB). TEM confirmed the association between osmium stained fat droplets,MDB formation, and cellular enlargement and suggested droplet-associated dilation of the endoplasmic reticulum. Conclusions: These results indicate a relationship between cellular ballooning, fat droplet accumulation, and cytoskeletal injury in NASH. We speculate that injury to multiple organelles, including fat droplets and endoplasmic reticulum, contribute to this characteristic finding. | pt_BR |
dc.language.iso | en | pt_BR |
dc.source | Journal of Hepatology | pt_BR |
dc.subject | Cellular ballooning | pt_BR |
dc.subject | Steatohepatitis | pt_BR |
dc.subject | NASH | pt_BR |
dc.subject | NAFLD | pt_BR |
dc.subject | Keratin 18 | pt_BR |
dc.subject | Fat staining | pt_BR |
dc.subject | Oil red O | pt_BR |
dc.title | Hepatocellular ballooning in NASH Stephen | pt_BR |
dc.title.alternative | JOURNAL OF HEPATOLOGY | pt_BR |
dc.type | Artigo de Periódico | pt_BR |
dc.identifier.number | v. 53, n. 4 | pt_BR |
dc.embargo.liftdate | 10000-01-01 | - |
Aparece nas coleções: | Artigo Publicado em Periódico (Faculdade de Medicina) |
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