https://repositorio.ufba.br/handle/ri/5601
metadata.dc.type: | Artigo de Periódico |
Title: | Hepatocellular ballooning in NASH Stephen |
Other Titles: | JOURNAL OF HEPATOLOGY |
Authors: | Caldwell, Stephen H. Ikura, Yoshihiro Dias, Daniela Isomoto, Kosuke |
metadata.dc.creator: | Caldwell, Stephen H. Ikura, Yoshihiro Dias, Daniela Isomoto, Kosuke |
Abstract: | Background & Aims: Hepatocellular ballooning is a key finding in nonalcoholic steatohepatitis (NASH). It is conventionally defined by hemotoxylin and eosin (H&E) staining showing enlarged cells with rarefied cytoplasm and recently by changes in the cytoskeleton. Fat droplets are emerging as important organelles in cell metabolism. To address a possible relation between fat droplets and ballooning, we studied fat staining, H&E, and keratin 18 staining in human NASH. Methods: Sequential staining and high resolution imaging were used to study freshly prepared cryo-sections from 10 patients with histologically confirmed steatohepatitis using oil red O for fat droplet identification, H&E to identify ballooning, and anti-K18 to confirm cytoskeletal changes. High resolution images were captured at each stage using the Aperio Scanscope. To provide ultrastructural correlation, glutaraldehyde-fixed specimens were studied using transmission electron microscopy (TEM) with serial sectioning for localization of ballooned cells by light microscopy and TEM in identical specimens. Results: Serial staining consistently demonstrated that hepatocellular ballooning is associated with fat droplet accumulation evident by oil red O positivity and depletion of cytoplasmic keratin 18 with K-18 positive Mallory–Denk bodies (MDB). TEM confirmed the association between osmium stained fat droplets,MDB formation, and cellular enlargement and suggested droplet-associated dilation of the endoplasmic reticulum. Conclusions: These results indicate a relationship between cellular ballooning, fat droplet accumulation, and cytoskeletal injury in NASH. We speculate that injury to multiple organelles, including fat droplets and endoplasmic reticulum, contribute to this characteristic finding. |
Keywords: | Cellular ballooning Steatohepatitis NASH NAFLD Keratin 18 Fat staining Oil red O |
URI: | http://www.repositorio.ufba.br/ri/handle/ri/5601 |
Issue Date: | 2010 |
Appears in Collections: | Artigo Publicado em Periódico (Faculdade de Medicina) |
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