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Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/5366
Tipo: Artigo de Periódico
Título: A piezoelectric immunosensor for Leishmania chagasi antibodies in canine serum
Título(s) alternativo(s): Analytical and Bioanalytical Chemistry
Autor(es): Jesus, Joilson Ramos
Carvalho, Kellyanne dos Anjos
Fonseca, Rosana Anita da Silva
Oliveira, Geraldo Gileno de Sá
Melo, Stella Maria Barrouin
Neves, Neuza Maria Alcântara
Dutra, Rosa Amália Fireman
Autor(es): Jesus, Joilson Ramos
Carvalho, Kellyanne dos Anjos
Fonseca, Rosana Anita da Silva
Oliveira, Geraldo Gileno de Sá
Melo, Stella Maria Barrouin
Neves, Neuza Maria Alcântara
Dutra, Rosa Amália Fireman
Abstract: The American visceral leishmaniasis is an important cause of morbidity and mortality in Brazil for both humans and dogs. Attempts to make a diagnosis of this disease need to be improved, especially in endemic areas, and in the tracking and screening of asymptomatic dogs, which are their main host in urban areas. A quartz crystal microbalance immunosensor for the diagnosis of the canine visceral leishmaniasis using a recombinant antigen of Leishmania chagasi (rLci2B-NH6) was devel- oped. The rLci2B-NH6 was tightly immobilized on a quartz crystal gold electrode by self-assembled monolayer based on short-chain length thiol. The strategy was the use of the antigen-histidine tail covalently linked to glutaral-dehyde performing a Schift base which permits a major exposure of epitopes and a reduced steric hindrance. The immunosensor showed good results regarding sensitivity and reproducibility, being able to distinguish positive and negative canine serum for L. chagasi. Furthermore, the immunosensor can be reused through exposure to sodium dodecyl sulfate solution, which promotes the dissociation of antigen–antibody binding, restoring the sensor surface with immobilized biologically active antigens for further analysis.
Palavras-chave: Leishmania chagasi
Immunosensor
Self-assembled monolayer
Piezoelectric
URI: http://www.repositorio.ufba.br/ri/handle/ri/5366
Data do documento: 2011
Aparece nas coleções:Artigo Publicado em Periódico (EMV)

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