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Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/5366
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dc.contributor.authorJesus, Joilson Ramos-
dc.contributor.authorCarvalho, Kellyanne dos Anjos-
dc.contributor.authorFonseca, Rosana Anita da Silva-
dc.contributor.authorOliveira, Geraldo Gileno de Sá-
dc.contributor.authorMelo, Stella Maria Barrouin-
dc.contributor.authorNeves, Neuza Maria Alcântara-
dc.contributor.authorDutra, Rosa Amália Fireman-
dc.creatorJesus, Joilson Ramos-
dc.creatorCarvalho, Kellyanne dos Anjos-
dc.creatorFonseca, Rosana Anita da Silva-
dc.creatorOliveira, Geraldo Gileno de Sá-
dc.creatorMelo, Stella Maria Barrouin-
dc.creatorNeves, Neuza Maria Alcântara-
dc.creatorDutra, Rosa Amália Fireman-
dc.date.accessioned2012-02-09T16:41:11Z-
dc.date.issued2011-
dc.identifier.issn1618-2650-
dc.identifier.urihttp://www.repositorio.ufba.br/ri/handle/ri/5366-
dc.descriptiontexto completo: restrito. p. 917–925pt_BR
dc.description.abstractThe American visceral leishmaniasis is an important cause of morbidity and mortality in Brazil for both humans and dogs. Attempts to make a diagnosis of this disease need to be improved, especially in endemic areas, and in the tracking and screening of asymptomatic dogs, which are their main host in urban areas. A quartz crystal microbalance immunosensor for the diagnosis of the canine visceral leishmaniasis using a recombinant antigen of Leishmania chagasi (rLci2B-NH6) was devel- oped. The rLci2B-NH6 was tightly immobilized on a quartz crystal gold electrode by self-assembled monolayer based on short-chain length thiol. The strategy was the use of the antigen-histidine tail covalently linked to glutaral-dehyde performing a Schift base which permits a major exposure of epitopes and a reduced steric hindrance. The immunosensor showed good results regarding sensitivity and reproducibility, being able to distinguish positive and negative canine serum for L. chagasi. Furthermore, the immunosensor can be reused through exposure to sodium dodecyl sulfate solution, which promotes the dissociation of antigen–antibody binding, restoring the sensor surface with immobilized biologically active antigens for further analysis.pt_BR
dc.language.isoenpt_BR
dc.sourceDOI 10.1007/s00216-011-5136-7pt_BR
dc.subjectLeishmania chagasipt_BR
dc.subjectImmunosensorpt_BR
dc.subjectSelf-assembled monolayerpt_BR
dc.subjectPiezoelectricpt_BR
dc.titleA piezoelectric immunosensor for Leishmania chagasi antibodies in canine serumpt_BR
dc.title.alternativeAnalytical and Bioanalytical Chemistrypt_BR
dc.typeArtigo de Periódicopt_BR
dc.identifier.numberv. 400.pt_BR
dc.embargo.liftdate10000-01-01-
Aparece nas coleções:Artigo Publicado em Periódico (EMV)

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