Aplicação da fibrina rica em plaquetas e a fotobiomodulação na regeneração óssea guaiada: estudo experimental em ratos

Abstract

Introduction: Bone loss can result from various etiological factors, such as traumas, pathological injuries, or tissue atrophy following the loss of dental units. The pursuit of improvements that assist or lead to bone repair to restore the anatomical pattern has increased. Autologous platelet-rich fibrin (PRF) membrane has been used to aid this process of bone neoformation, demonstrating good results, as has low-level laser therapy (LLLT).Objective: To investigate the effect of LLLT (λ = 830 nm), with the use of the PRF membrane, on bone neoformation. Methodology: This membrane was obtained from intracardiac puncture of donor rats. A bone defect was created in the calvaria of 48 Wistar rats, which were divided into four groups: D (bone defect), DL (bone defect and laser), DPRF (bone defect and PRF), and DPRFL (defect, PRF, and laser). Six animals from each group were euthanized after 30 and 60 days and processed histologically. Total cell count, presence of angiogenesis, and area of formed bone repair were evaluated. Results: In the analysis between groups, there was a significant difference in the total number of cells between groups D and DPRFL after 30 (p***< 0.001) and 60 days (p**< 0.01), as well as a greater presence of angiogenesis (p**< 0.01). Although the difference was not significant, group DPRFL showed a greater area of repair in bone defect created after 30 days. Conclusion: PRF had a positive effect on bone neoformation, and when associated with LLLT, it showed greater vertical bone thickness.