Avaliação da atividade imunomoduladora do extrato etanólico de Physalis angulata em cultura de linhagem PC12

Abstract

Physalis angulata is a plant that can be found in tropical and subtropical regions, where it is used in folk medicine for the treatment of numerous diseases. Several studies using different components of P. angulata proved anti-inflammatory, antiparasitic, antioxidant and protective action. Therefore, the present work sought to evaluate the effects of the crude ethanolic extract of P. angulata (EEPA) in cell cultures of the PC12 lineage. EEPA was produced from the stems of P. angulata. The cytotoxicity of different concentrations of EEPA was determined using the MTT assay. To evaluate cell morphology and cell proliferation, Rosenfeld dye was used. ImageJ v.1.53e software was used to count the cells. To verify the immune response, nitric oxide levels were analyzed, determined by measuring nitrite using the Griess method. The results were analyzed by the statistical program GraphPadPrism 7.0 and the data were expressed as mean ± standard deviation of the mean of the evaluated parameters. Treatment with EEPA at concentrations 0.5; 1; 5 μg/mL suggest that there was no cytotoxicity or reduction in the number of PC12 cells. The extract caused morphological changes in cells at all concentrations. NO production was reduced at all concentrations, mainly at 0.5μg/mL. The data obtained in our work suggest that EEPA can negatively modulate NO production and change the morphology of PC12 cells, promoting the appearance of processes suggestive of neurites.