https://repositorio.ufba.br/handle/ri/16544
Tipo: | Artigo de Periódico |
Título: | Schistosoma Antigens Downmodulate the in vitro Inflammatory Response in Individuals Infected with Human T Cell Lymphotropic Virus Type 1 |
Título(s) alternativo(s): | Neuroimmunomodulation |
Autor(es): | Lima, Luciane Mota Santos, Silvane Maria Braga Oliveira, Ricardo Riccio Cardoso, Luciana Santos Oliveira, Sérgio Costa Góes, Alfredo Miranda Loukas, Alex Carvalho Filho, Edgar Marcelino de Araujo, Maria Ilma Andrade Santos |
Autor(es): | Lima, Luciane Mota Santos, Silvane Maria Braga Oliveira, Ricardo Riccio Cardoso, Luciana Santos Oliveira, Sérgio Costa Góes, Alfredo Miranda Loukas, Alex Carvalho Filho, Edgar Marcelino de Araujo, Maria Ilma Andrade Santos |
Abstract: | Human T cell lymphotropic virus type 1 (HTLV-1) is the causal agent of HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). While the immune response to HTLV-1 infection is polarized to the Th1-type, chronic helminth infections drive the Th2- and T regulatory-type, and are able to downregulate the inflammatory response in some autoimmune diseases. Objective: To evaluate whether Schistosoma spp. antigens alter the in vitro cytokine response in HTLV-1 infection. Methods: The recombinant Schistosoma antigens Sm29 and ShTSP2 (tetraspanin) and PIII, a fraction of the Schistosoma mansoni adult worm antigen were added to peripheral blood mononuclear cell (PBMC) cultures of HTLV-1-infected individuals and the levels of interferon (IFN)-γ and interleukin (IL)-10 in the supernatants were measured using the ELISA sandwich technique. Results: Compared to the levels of cytokine in nonstimulated cultures, the levels of IFN-γ were reduced in 50, 47 and 50% of patients by the presence of Sm29, ShTsp2 and PIII, respectively. The downregulation of IFN-γ production in the presence of Sm29 antigen was observed mainly in subjects who had lower basal levels of this cytokine. The levels of IL-10, however, increased by the addition of the three antigens in the cultures in 74, 62 and 44% of individuals, respectively. In addition, there was a decrease in the ratio of IFN-γ/IL-10 levels in cultures stimulated with Sm29 and ShTSP2 when compared to nonstimulated ones. Conclusions: The Schistosoma spp. antigens used in this study were able to downmodulate IFN-γ production in vitro in HTLV-1 infection. This may be associated with the increased levels of IL-10 induced by the antigens. |
Palavras-chave: | Human T cell lymphotropic virus type 1 Schistosoma spp. antigens Interferon-γ Interleukin-10 |
Tipo de Acesso: | Acesso Aberto |
URI: | http://repositorio.ufba.br/ri/handle/ri/16544 |
Data do documento: | 2013 |
Aparece nas coleções: | Artigo Publicado em Periódico (FAR) |
Arquivo | Descrição | Tamanho | Formato | |
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Luciane Mota Lima | 489,68 kB | Adobe PDF | Visualizar/Abrir |
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