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https://repositorio.ufba.br/handle/ri/14888
metadata.dc.type: | Artigo de Periódico |
Title: | An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation |
Other Titles: | Ciência e Tecnologia de Alimentos |
Authors: | Roque, Milton Ricardo de Abreu Paulo, Elinalva Maciel Vasconcelos, Murilo Pinho Oliveira, Ivelise Santiago Affe, Helen Michelle de Jesus Nascimento, Rosely Melo, Itamar Soares de Assis, Sandra Aparecida de |
metadata.dc.creator: | Roque, Milton Ricardo de Abreu Paulo, Elinalva Maciel Vasconcelos, Murilo Pinho Oliveira, Ivelise Santiago Affe, Helen Michelle de Jesus Nascimento, Rosely Melo, Itamar Soares de Assis, Sandra Aparecida de |
Abstract: | The accumulation of exopolysaccharides (EPS) produced by microorganisms occurs in the presence of excess substrate and limiting conditions of elements that are essential to growth, such as nitrogen, phosphorus, sulfur, and magnesium. The presence of EPS produced by bacterial cells contributes to slime colonies formation in solid medium and increased viscosity in liquid medium. This paper proposes an alternative method for screening EPS-producing lactic acid bacteria using solid medium-containing discs of filter paper that are saturated with active cultures. The screening was carried out under different culture conditions varying the type of sugar, pH, and temperature. EPS production was visualized by the presence of mucoid colonies on the discs, which was confirmed by the formation of a precipitate when part of this colony was mixed with absolute alcohol. The established conditions for obtaining a high number of isolates producing EPS were 10% sucrose, pH 7.5 and 28 ºC. This method proved to be effective and economical because several strains could be tested on the same plate, with immediate confirmation. |
Keywords: | Screening Exopolysaccharides Lactic acid bacteria Triagem Exopolissacarídeos Bactérias lácticas |
metadata.dc.publisher.country: | Brasil |
metadata.dc.rights: | Acesso Aberto |
URI: | http://repositorio.ufba.br/ri/handle/ri/14888 |
Issue Date: | 2012 |
Appears in Collections: | Artigo Publicado em Periódico (ICS) |
Files in This Item:
File | Description | Size | Format | |
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Milton Ricardo de Abreu Roque.pdf | 3,16 MB | Adobe PDF | View/Open |
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