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Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/14600
Tipo: Artigo de Periódico
Título: Multiplex PCR assay for identification of Corynebacterium pseudotuberculosis from pure cultures and for rapid detection of this pathogen in clinical samples
Título(s) alternativo(s): Journal of Medical Microbiology
Autor(es): Pacheco, Luis Gustavo Carvalho
Pena, Roberta R.
Castro, Thiago Luiz de Paula
Dorella, Fernanda Alves
Bahia, Robson Cerqueira
Carminati, Renato
Nascimento, Roberto José Meyer
Autor(es): Pacheco, Luis Gustavo Carvalho
Pena, Roberta R.
Castro, Thiago Luiz de Paula
Dorella, Fernanda Alves
Bahia, Robson Cerqueira
Carminati, Renato
Nascimento, Roberto José Meyer
Abstract: Corynebacterium pseudotuberculosis is the aetiological agent of caseous lymphadenitis (CLA), a debilitating disease of sheep and goats. Accurate diagnosis of CLA primarily relies on microbiological examination, followed by biochemical identification of isolates. In an effort to facilitate C. pseudotuberculosis detection, a multiplex PCR (mPCR) assay was developed targeting three genes of this bacterium: the 16S rRNA gene, rpoB and pld. This method allowed efficient identification of 40 isolates of this bacterium that had been identified previously by biochemical testing. Analysis of taxonomically related species did not generate the C. pseudotuberculosis mPCR amplification profile, thereby demonstrating the assay's specificity. As little as 1 pg of C. pseudotuberculosis genomic DNA was detected by this mPCR assay, demonstrating the sensitivity of the method. The detection limit in clinical samples was estimated to be 103 c.f.u. C. pseudotuberculosis could be detected directly in pus samples from infected sheep and goats (n=56) with a high diagnostic sensitivity (94.6 %). The developed assay significantly improves rapid C. pseudotuberculosis detection and could supersede bacteriological culture for microbiological and epidemiological diagnosis of CLA.
Palavras-chave: CLA, caseous lymphadenitis
mPCR, multiplex PCR
PLD, phospholipase D
Tipo de Acesso: Acesso Aberto
URI: http://repositorio.ufba.br/ri/handle/ri/14600
Data do documento: 2007
Aparece nas coleções:Artigo Publicado em Periódico (Biologia)

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