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Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/14600
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dc.contributor.authorPacheco, Luis Gustavo Carvalho-
dc.contributor.authorPena, Roberta R.-
dc.contributor.authorCastro, Thiago Luiz de Paula-
dc.contributor.authorDorella, Fernanda Alves-
dc.contributor.authorBahia, Robson Cerqueira-
dc.contributor.authorCarminati, Renato-
dc.contributor.authorNascimento, Roberto José Meyer-
dc.creatorPacheco, Luis Gustavo Carvalho-
dc.creatorPena, Roberta R.-
dc.creatorCastro, Thiago Luiz de Paula-
dc.creatorDorella, Fernanda Alves-
dc.creatorBahia, Robson Cerqueira-
dc.creatorCarminati, Renato-
dc.creatorNascimento, Roberto José Meyer-
dc.date.accessioned2014-02-13T17:49:33Z-
dc.date.issued2007-
dc.identifier.issn0022-2615-
dc.identifier.urihttp://repositorio.ufba.br/ri/handle/ri/14600-
dc.descriptionTexto completo: acesso restrito. p. 480-486pt_BR
dc.description.abstractCorynebacterium pseudotuberculosis is the aetiological agent of caseous lymphadenitis (CLA), a debilitating disease of sheep and goats. Accurate diagnosis of CLA primarily relies on microbiological examination, followed by biochemical identification of isolates. In an effort to facilitate C. pseudotuberculosis detection, a multiplex PCR (mPCR) assay was developed targeting three genes of this bacterium: the 16S rRNA gene, rpoB and pld. This method allowed efficient identification of 40 isolates of this bacterium that had been identified previously by biochemical testing. Analysis of taxonomically related species did not generate the C. pseudotuberculosis mPCR amplification profile, thereby demonstrating the assay's specificity. As little as 1 pg of C. pseudotuberculosis genomic DNA was detected by this mPCR assay, demonstrating the sensitivity of the method. The detection limit in clinical samples was estimated to be 103 c.f.u. C. pseudotuberculosis could be detected directly in pus samples from infected sheep and goats (n=56) with a high diagnostic sensitivity (94.6 %). The developed assay significantly improves rapid C. pseudotuberculosis detection and could supersede bacteriological culture for microbiological and epidemiological diagnosis of CLA.pt_BR
dc.language.isoenpt_BR
dc.rightsAcesso Abertopt_BR
dc.sourcehttp://dx.doi.org/10.1099/jmm.0.46997-0pt_BR
dc.subjectCLA, caseous lymphadenitispt_BR
dc.subjectmPCR, multiplex PCRpt_BR
dc.subjectPLD, phospholipase Dpt_BR
dc.titleMultiplex PCR assay for identification of Corynebacterium pseudotuberculosis from pure cultures and for rapid detection of this pathogen in clinical samplespt_BR
dc.title.alternativeJournal of Medical Microbiologypt_BR
dc.typeArtigo de Periódicopt_BR
dc.identifier.numberv. 56, n. 4pt_BR
dc.embargo.liftdate10000-01-01-
Aparece nas coleções:Artigo Publicado em Periódico (Biologia)

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