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metadata.dc.type: | Artigo de Periódico |
Title: | Enzymatic activities and protein profile of latex from Calotropis procera |
Other Titles: | Plant Physiology and Biochemistry |
Authors: | Freitas, Cleverson Diniz T. Oliveira, Jefferson Soares Miranda, Maria Raquel A. Macedo, Nívea Maria R. Sales, Maurício Pereira Villas Boas, Laurival A. Ramos, Márcio Viana |
metadata.dc.creator: | Freitas, Cleverson Diniz T. Oliveira, Jefferson Soares Miranda, Maria Raquel A. Macedo, Nívea Maria R. Sales, Maurício Pereira Villas Boas, Laurival A. Ramos, Márcio Viana |
Abstract: | The laticifer fluid of Calotropis procera is rich in proteins and there is evidence that they are involved in the pharmacological properties of the latex. However, not much is known about how the latex-containing proteins are produced or their functions. In this study, laticifer proteins of C. procera were pooled and examined by 1D and 2D electrophoresis, masses spectrometry (MALDI-TOF) and characterized in respect of proteolytic activity and oxidative enzymes. Soluble laticifer proteins were predominantly composed of basic proteins (PI > 6.0) with molecular masses varying between 5 and 95 kDa. Proteins with a molecular mass of approximately 26,000 Da were more evident. Strong anti-oxidative activity of superoxide dismutase (EC 1.15.1.1) (1007.74 ± 91.89 U g−1 DM) and, to a lesser extent ascorbate peroxidase (EC 1.11.1.1) (0.117d ± 0.013 μMol H2O2 g−1 min−1), were detected. However, catalase (EC 1.11.1.6) was absent. The strong proteolytic activities of laticifer proteins from C. procera were shown to be shared by at least four distinct cysteine proteinases (EC 3.4.22.16) that were isolated by gel filtration chromatography. Serine and metaloproteinases were not detected and aspartic proteinase activities were barely visible. Chitinases (EC 3.2.1.14) were also isolated in a chitin column and their activities quantified. The presence of these enzymatic activities in latex from C. procera may confirm their involvement in resistance to phytopathogens and insects, mainly in its leaves where the latex circulates abundantly. |
Keywords: | Latex Proteolytic activity Oxidative enzymes Plant defense |
metadata.dc.rights: | Acesso Aberto |
URI: | http://repositorio.ufba.br/ri/handle/ri/14450 |
Issue Date: | 2007 |
Appears in Collections: | Artigo Publicado em Periódico (Biologia) |
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File | Description | Size | Format | |
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1-s2.0-S0981942807001611-main.pdf | 870,82 kB | Adobe PDF | View/Open |
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