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https://repositorio.ufba.br/handle/ri/14421
metadata.dc.type: | Artigo de Periódico |
Title: | Diagnosis of canine brucellosis by ELISA using an antigen obtained from wild Brucella canis |
Other Titles: | Research in Veterinary Science |
Authors: | Melo, Stella Maria Barrouin Poester, Fernando Padilla Ribeiro, Marcos Borges Alcântara, Adriano Costa de Aguiar, Paulo Henrique Palis Nascimento, Ivana Lúcia de Oliveira Schaer, Robert Eduard Nascimento, Roberto José Meyer Freire, Songelí Menezes |
metadata.dc.creator: | Melo, Stella Maria Barrouin Poester, Fernando Padilla Ribeiro, Marcos Borges Alcântara, Adriano Costa de Aguiar, Paulo Henrique Palis Nascimento, Ivana Lúcia de Oliveira Schaer, Robert Eduard Nascimento, Roberto José Meyer Freire, Songelí Menezes |
Abstract: | An indirect ELISA test was developed for the diagnosis of Brucella canis infection in dogs. A bacterial whole cell extract was used as a solid phase antigen, using B. canis isolated from an infected animal. Sera from culture-positive and healthy negative animals were used as internal reference controls. The cut-off point was determined by a mathematical formula for a statistically valid value, which defined the upper prediction limit, based on the upper tail of the t-distribution of 21 negative control sera readings, for the confidence level of 99.5%. The sensitivity and specificity of the ELISA test were 95% and 91%, respectively. The ELISA test showed a significant concordance index (K = 0.84) with the agar gel immunodiffusion test. The reliability of the ELISA for the detection of infected animals was established by a double blind study testing 280 sera provided by serum banks from different diagnostic and research institutions and analyzed by ROC Curve. |
Keywords: | Brucella canis ELISA Serodiagnosis Canine brucellosis |
metadata.dc.rights: | Acesso Aberto |
URI: | http://repositorio.ufba.br/ri/handle/ri/14421 |
Issue Date: | 2007 |
Appears in Collections: | Artigo Publicado em Periódico (EMV) |
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1-s2.0-S0034528807000501-main.pdf | 388,35 kB | Adobe PDF | View/Open |
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