https://repositorio.ufba.br/handle/ri/13682
Tipo: | Artigo de Periódico |
Título: | Azurocidin, a Natural Antibiotic from Human Neutrophils: Expression, Antimicrobial Activity, and Secretion |
Título(s) alternativo(s): | Protein Expression and Purification |
Autor(es): | Almeida, Roque Pacheco de Vanet, Anne Witko-Sarsat, Veronique Melchior, Maxine McCabe, Denise Gabay, Joelle E. |
Autor(es): | Almeida, Roque Pacheco de Vanet, Anne Witko-Sarsat, Veronique Melchior, Maxine McCabe, Denise Gabay, Joelle E. |
Abstract: | The azurophil granules of human PMN contain four antibiotic proteins, the serprocidins, which have extensive homology to one another and to serine proteases. Azurocidin, a member of this family, is a 29-kDa glycoprotein with broad spectrum antimicrobial activity and chemotactic activity toward monocytes. Insect cells transfected with a baculovirus vector carrying azurocidin cDNA produced a recombinant azurocidin protein. We purified the recombinant azurocidin protein from the culture medium of the infected cells and showed that it retained the antimicrobial activity of the native neutrophil-derived molecule. In addition, we present evidence that a 49-amino-acid region of the recombinant azurocidin protein is required for its secretion from insect cells. |
Editora / Evento / Instituição: | Protein Expression and Purification |
Tipo de Acesso: | Acesso Aberto |
URI: | http://repositorio.ufba.br/ri/handle/ri/13682 |
Data do documento: | 1996 |
Aparece nas coleções: | Artigo Publicado em Periódico (Faculdade de Medicina) |
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