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https://repositorio.ufba.br/handle/ri/13114
metadata.dc.type: | Artigo de Periódico |
Title: | Validation of a high-performance liquid chromatography method for the determination of (-)-α-bisabolol from particulate systems |
Other Titles: | Biomedical Chromatography |
Authors: | São Pedro, André Detoni, Cássia Britto Ferreira, Domingos Albuquerque, Elaine Christine de Magalhães Cabral Sarmento, Bruno |
metadata.dc.creator: | São Pedro, André Detoni, Cássia Britto Ferreira, Domingos Albuquerque, Elaine Christine de Magalhães Cabral Sarmento, Bruno |
Abstract: | A reversed-phase high performance liquid chromatography method has been developed and validated for determination and quantitation of the natural sesquiterpene (−)-α-bisabolol. Furthermore the application of the method was done by characterization of chitosan milispheres and liposomes entrapping Zanthoxylum tingoassuiba essential oil, which contains appreciable amount of (−)-α-bisabolol. A reversed-phase C18 column and gradient elution was used with the mobile phase composed of (A) acetonitrile–water–phosphoric acid (19:80:1) and (B) acetonitrile. The eluent was pumped at a flow rate of 0.8 mL/min with UV detection at 200 nm. In the range 0.02–0.64 mg/mL the assay showed good linearity (R2 = 0.9999) and specificity for successful identification and quantitation of (−)-α-bisabolol in the essential oil without interfering peaks. The method also showed good reproducibility, demonstrating inter-day and intra-day precision based on relative standard deviation values (up to 3.03%), accuracy (mean recovery of 100.69% ± 1.05%) and low values of detection and quantitation limits (0.0005 and 0.0016 mg/mL, respectively). The method was also robust for showing a recovery of 98.81% under a change of solvent in standard solutions. The suitability of the method was demonstrated by the successful determination of association efficiency of the (−)-α-bisabolol in chitosan milispheres and liposomes. |
Keywords: | Chitosan Liposomes (−)-α-bisabolol Method validation RP-HPLC |
URI: | http://www.repositorio.ufba.br/ri/handle/ri/13114 |
Issue Date: | 2009 |
Appears in Collections: | Artigo Publicado em Periódico (FAR) |
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