Please use this identifier to cite or link to this item:
https://repositorio.ufba.br/handle/ri/12449
metadata.dc.type: | Artigo de Periódico |
Title: | Virulent or avirulent (dhfr-tsÿ) Leishmania major elicit predominantly a type-1 cytokine response by human cells in vitro |
Other Titles: | Clinical and Experimental Immunology |
Authors: | Brodskyn, Claudia Ida Beverley, S. M. Titus, R. G. |
metadata.dc.creator: | Brodskyn, Claudia Ida Beverley, S. M. Titus, R. G. |
Abstract: | In this study we have compared the immune response of normal human cells cultured in vitro to two virulent strains of Leishmania major (CC1 and LV39), and to an avirulent vaccine strain (dhfr-tsÿ) made by targeted deletion of the essential gene DHFR-TS. We utilized an in vitro system in which naive T cells from normal human donors were primed with autologous Leishmania-infected macrophages. All three parasites infected macrophages and transformed into amastigotes within the cells. However, whereas LV39 and CC1 replicated in macrophages, dhfr-tsÿ did not. When peripheral blood lymphocytes (PBL) were stimulated with autologous macrophages infected with any of the three parasites, the lymphocytes produced a type-1-biased cytokine response. Finally, addition of IL-12 during the ®rst stimulation period increased the production of interferon-gamma but decreased IL-5 secretion. On the other hand, anti-IL-12 resulted in the opposite effect. |
Keywords: | Leishmania major Macrophages Th1 Th2 IL-12 |
Publisher: | Clinical and Experimental Immunology |
URI: | http://www.repositorio.ufba.br/ri/handle/ri/12449 |
Issue Date: | 2000 |
Appears in Collections: | Artigo Publicado em Periódico (ICS) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
22222222222222222.pdf Restricted Access | 166,24 kB | Adobe PDF | View/Open Request a copy |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.