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https://repositorio.ufba.br/handle/ri/12109
metadata.dc.type: | Artigo de Periódico |
Título : | Flow cytometric screening for anti-leishmanials in a human macrophage cell line |
Otros títulos : | Experimental Parasitology |
Autor : | Mehta, Sanjay R. Xing, Quan Zhang Badaró, Roberto José da Silva Spina, Celsa Day, John Kwang, Poo Chang Schooley, Robert T. |
metadata.dc.creator: | Mehta, Sanjay R. Xing, Quan Zhang Badaró, Roberto José da Silva Spina, Celsa Day, John Kwang, Poo Chang Schooley, Robert T. |
Resumen : | High-throughput drug screening methods against the intracellular stage of Leishmania have been facilitated by the development of in vitro models of infection. The use of cell lines rather than primary cells facilitates these methods. Peripheral blood mononuclear cell (PBMC) derived macrophages and THP-1 cells were infected with stationary phase egfp transfected Leishmania amazonensis parasites and then treated with anti-leishmanial compounds. Drug activity was measured using a flow cytometric approach, and toxicity was assessed using either the MTT assay or trypan blue dye exclusion. Calculated EC50’s for amphotericin B, sodium stibogluconate, and miltefosine were 0.1445 ± 0.0005 μg/ml, 0.1203 ± 0.018 mg/ml, and 26.71 μM using THP-1 cells, and 0.179 ± 0.035 μg/ml, 0.1948 ± 0.0364 mg/ml, and 13.77 ± 10.74 μM using PBMC derived macrophages, respectively. We conclude that a flow cytometric approach using egfp transfected Leishmania species can be used to evaluate anti-leishmanial compounds against the amastigote stage of the parasite in THP-1 cells with excellent concordance to human PBMC derived macrophages. |
Palabras clave : | Leishmania amazonensis Parasite Amastigote Drug screening THP-1 Flow cytometry |
URI : | http://www.repositorio.ufba.br/ri/handle/ri/12109 |
Fecha de publicación : | 2010 |
Aparece en las colecciones: | Artigo Publicado em Periódico (Faculdade de Medicina) |
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1-s2.0-S001448941000202X-main.pdf Restricted Access | 315,8 kB | Adobe PDF | Visualizar/Abrir Request a copy |
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