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https://repositorio.ufba.br/handle/ri/11517
metadata.dc.type: | Artigo de Periódico |
Título : | Early Detection of goats infected with Lentivirus Small Ruminant virus by ELISA assay |
Otros títulos : | Revista de Ciências Médicas e Biológicas |
Autor : | Sardi, Silvia Inês Torres, Julianna Alves Brandão, Camila Fonseca Lopes Tigre, Dellane Martins Campos, Gubio Soares |
metadata.dc.creator: | Sardi, Silvia Inês Torres, Julianna Alves Brandão, Camila Fonseca Lopes Tigre, Dellane Martins Campos, Gubio Soares |
Resumen : | Many established protocols are available to produce antigens for ELISA tests but expensive equipments, the purification methods or recombinant technologies are not always available to veterinary diagnostic laboratories. The aim of this work was to develop an alternative ELISA test to detect antibodies against Lentivirus Small Ruminant Virus (Caprine arthritis-encephalitis virus-CAEV) in goats, simple to elaborate with a low cost of production. The antigen was obtain from a whole cellular lysates of goat synovial membrane cells (GSMC) infected with CAEV and treated with SDS 0,1%. The wholeviral antigen was capable of detecting antibodies against several viral proteins including the p28 kDa (viral capsid), the p33 kDa, the p44 kDa (capsid precursors) and p97 kDa (transmembrane glycoprotein). The comparison with others assays as indirect immunofluorescence showed a high correlation with the ELISA results and the polimerasse chain reaction (PCR) demonstrated that most of the goat seropositives by ELISA, had proviral DNA in the milk cells. In conclusion, the viral antigen obtained from GSMC was simple to elaborate, low cost of production. The ELISA test was capable of detecting antibodies against CAEV, to detect early infection. |
Palabras clave : | Lentiviruses Ovine-Caprine Enzyme-Linked Immunosorbent Assay Diagnosis Lentivirus Ovinos-Caprinos Ensaio de Imunoadsorção Enzimática Diagnóstico |
Editorial : | Revista de Ciências Médicas e Biológicas |
URI : | http://www.repositorio.ufba.br/ri/handle/ri/11517 |
Fecha de publicación : | 2012 |
Aparece en las colecciones: | Artigo Publicado em Periódico (ICS) |
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Fichero | Descripción | Tamaño | Formato | |
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