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https://repositorio.ufba.br/handle/ri/7696| Tipo: | Artigo de Periódico |
| Título: | Three-phase partitioning as an efficient method for extraction/concentration of immunoreactive excreted–secreted proteins of Corynebacterium pseudotuberculosis |
| Título(s) alternativo(s): | Protein Expression and Purification |
| Autor(es): | Paule, B. J. A. Nascimento, Roberto José Meyer Costa, L. F. Moura Bahia, Robson Cerqueira Carminati, R. Regis, Lia Fernandes |
| Autor(es): | Paule, B. J. A. Nascimento, Roberto José Meyer Costa, L. F. Moura Bahia, Robson Cerqueira Carminati, R. Regis, Lia Fernandes |
| Abstract: | The three-phase partitioning (TPP) technique was used upstream to isolate/concentrate secreted proteins from Corynebacterium pseudotuberculosis cultured in a complex liquid medium. Several parameters of the TPP technique (15, 30, or 60% ammonium sulfate concentration; 4.0, 5.5, or 7.0 pH; and primary (n) or tertiary (t)-butanol solvent isomer) were varied to determine the optimal recovery of serologically and cellularly immunoreactive extracted proteins. A TPP extraction made with 30% ammonium sulfate and an initial pH of 4.0 gave the best humoral and cellular immunoreactivity of caseous lymphadenitis infected goats. In particular, two immunogenic secreted (16 and 125 kDa) proteins, which had not been found by other extraction methods, were identified. |
| Palavras-chave: | Corynebacterium pseudotuberculosis Excreted–secreted proteins Three-phase partitioning |
| Editora / Evento / Instituição: | Elsevier |
| URI: | http://www.repositorio.ufba.br/ri/handle/ri/7696 |
| Data do documento: | 2004 |
| Aparece nas coleções: | Artigo Publicado em Periódico (Faculdade de Medicina) |
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