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|Title: ||Evaluation of factors that can modify platelet-rich plasma properties|
|Other Titles: ||ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY|
|Authors: ||Andrade, Miguel Gustavo Setúbal|
Brandão, Claudio José de Freitas
Sá, Camila Neves
Bittencourt, Thereza Cristina Borio dos Santos Calmon de
|Issue Date: ||2008|
|Abstract: ||Objective The aim of this article is to discuss a protocol for obtaining platelet-rich plasma (PRP) and evaluate which
factors, derived from its preparation method and from whole blood, modify PRP cytometry and coagulation time. Study design Whole blood, harvested from 50 rabbits, was centrifuged at 300g for 10 minutes. Supernatant was recentrifuged at 5000g for 5 minutes. PRP was clotted with calcium chloride. Whole blood and PRP cytometry were
obtained through automatic measurement. The amount of erythrocyte- and platelet-poor plasma drawn from whole blood was measured. Hematocrit, platelet and leukocyte count, mean corpuscular volume (MCV) and mean platelet volume (MPV), mean, standard deviation, and median were also calculated at whole blood and PRP. PRP coagulation
time was also analyzed. Mean values between groups were analyzed using Student t test. Correlations were evaluated using Pearson’s correlation coefficient. The significance level was set at P .05. A linear regression was performed to investigate the relationship among the correlated variables.
Results From whole blood, 2.68 mL of erythrocytes and 5.72 mL of platelet-poor plasma (PPP) were removed. PRP platelet count was 2,324,080 cells/ L. Whole blood hematocrit influenced the amount of cells and PPP removed, as well as PRP platelet count. PRP platelet count was dependent on whole blood hematocrit and platelet count, and does not interfere in PRP coagulation time. A linear interaction was confirmed between the variables that presented
significant Pearson correlation.
Conclusions The protocol evaluated produces a good PRP. Whole-blood parameters can predict PRP features. Wholeblood
hematocrit is an important variable for PRP preparation and PRP cytometry characterization. PRP platelet count
is dependent upon whole-blood platelet count. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008;105:e5-e12)|
|Description: ||Acesso restrito: Texto completo. p. 5-12|
|Appears in Collections:||Artigos Publicados em Periódicos (Medicina)|
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