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Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/6088
Tipo: Artigo de Periódico
Título: Monocrotaline pyrrol is cytotoxic and alters the patterns of GFAP expression on astrocyte primary cultures
Título(s) alternativo(s): Toxicology in Vitro
Autor(es): Barreto, R. A.
Sousa, C. S.
Silva, V. D. A.
Silva, A. R.
Velozo, Eudes da Silva
Cunha, Silvio do Desterro
Costa, Maria de Fátima Dias
El-Bachá, Ramon dos Santos
Costa, Silvia Lima
Autor(es): Barreto, R. A.
Sousa, C. S.
Silva, V. D. A.
Silva, A. R.
Velozo, Eudes da Silva
Cunha, Silvio do Desterro
Costa, Maria de Fátima Dias
El-Bachá, Ramon dos Santos
Costa, Silvia Lima
Abstract: Dehydromonocrotaline (DHMC) is the main monocrotaline active cytochrome P450’s metabolite, and has already been assessed in the CNS of experimentally intoxicated rats. DHMC effects were here investigated toward rat astroglial primary cultures regarding cytotoxicity, morphological changes and regulation of GFAP expression. Cells, grown in DMEM supplemented medium, were treated with 0.1–500 lM DHMC, during 24- and 72-h. According to MTT and LDH tests, DHMC was toxic to astrocytes after 24-h exposure at 1 lM, and induced membrane damages at 500 lM. Rosenfeld dying showed hypertrophic astrocytes after 72-h exposure to 0.1–1 lM DHMC. GFAP immunocytochemistry and western immunoblot revealed an increase of GFAP labelling and expression, suggesting an astrogliotic reaction to low concentrations of DHMC. At higher concentrations (10–500 lM), astrocytes shrank their bodies and retracted their processes, presenting a more polygonal phenotype and a weaker expression on GFAP labelling Nuclear chromatin staining by Hoechst-33258 dye, revealed condensed and fragmented chromatin in an important proportion (±30%) of the astrocytes exposed to 100–500 lM DHMC, suggesting signs of apoptosis. Our results confirm a cytotoxic and dose-dependant effect of DHMC on cultures of rat cortical astrocytes, leading to apoptotic figures. These effects might be related to the neurological damages and clinical signs observed in animals intoxicated by Crotalaria.
Palavras-chave: Dehydromonocrotaline
Pyrrolizidine alkaloid
Astrocytes
GFAP
URI: http://www.repositorio.ufba.br/ri/handle/ri/6088
Data do documento: 2008
Aparece nas coleções:Artigo Publicado em Periódico (FAR)

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