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|Title: ||RE-PCR variability and toxigenic profile of food poisoning, foodborne and soil-associated Bacillus cereus isolates from Brazil|
|Other Titles: ||International Journal of Food Microbiology|
|Authors: ||Guimarães, Alaíse Gil|
Santos, C. A.
Almeida, F. S.
Abrahão, Wanda Moscalewski
Arantes, O. M. N.
Vilas-Bôas, Gislayne Trindade
|Keywords: ||Bacillus cereus;Food poisoning outbreaks;Foodborne;Soil;Virulence factors;RE-PCR|
|Issue Date: ||2011|
|Abstract: ||Twenty-three Bacillus cereus isolates from food poisoning outbreaks associated with a diarrheal-type syndrome, fourteen foodborne isolates not associated with food poisoning and fifteen isolates from Brazilian soil samples were analyzed for the presence and genetic diversity (by RE-PCR) of the virulence genes ces (emetic toxin, cereulide), plcR–papR (pleiotropic regulator PlcR and peptide PapR), nheA (a component of
the NHE complex), bceT (diarrheal enterotoxin bc-D-ENT), gyrB (B subunit of DNA gyrase), cytK-2 (necrotic enterotoxin cytotoxin K-2), and plcA (phosphatidylcholine-specific phospholipase C). Additionally, these isolates were phenotypically characterized for motility, hemolytic and lecithinase activities, as well as HBL enterotoxin
production. The group of isolates associated with food poisoning had the highest occurrence of the phenotypically analyzed factors and the most frequent occurrence and highest genetic diversity of the
plcR–papR, nheA, bceT, cytK-2, plcA, and gyrB genes. An analysis of molecular variance (AMOVA), in which all loci were analyzed, demonstrated that the genetic variation intragroup of isolates (92%) was significantly higher than that intergroup (8%) (Pb0.05). These results were corroborated by an analysis of the genetic differentiation between the groups, which was low/moderate, the result of a high degree of allele sharing. Our results suggest that B. cereus isolates with the potential to cause food poisoning outbreaks do not have a specific genetic profile characterized by the presence of a particular gene or allele among the genes assessed. On the contrary, different combinations of genes encoding virulence factors may be present in different isolates of B. cereus that potentially cause food poisoning outbreaks.|
|Description: ||texto completo: acesso restrito. p.277–283.|
|Appears in Collections:||Artigos Publicados em Periódicos (Nutrição)|
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