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Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/3071
Tipo: Artigo de Periódico
Título: Human T cell lymphotropic virus type 1 (HTLV-1) proviral load of HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients according to new diagnostic criteria of HAM/TSP
Título(s) alternativo(s): J Med Virol
Autor(es): Grassi, Maria Fernanda Rios
Olavarria, Viviana Nilla
Kruschewsky, Ramon de Almeida
Mascarenhas, Rita Elizabeth
Dourado, Maria Inês Costa
Correia, Luis Cláudio Lemos
Costa, Carlos Maurıcio de Castro
Castro, Bernardo Galvão
Autor(es): Grassi, Maria Fernanda Rios
Olavarria, Viviana Nilla
Kruschewsky, Ramon de Almeida
Mascarenhas, Rita Elizabeth
Dourado, Maria Inês Costa
Correia, Luis Cláudio Lemos
Costa, Carlos Maurıcio de Castro
Castro, Bernardo Galvão
Abstract: A high human T-cell lymphotropic virus type 1 (HTLV-1) proviral load is described in HTLV-1-associated diseases, especially HAM/TSP. However, the cut-off value to define high levels of HTLV-1 proviral load is not well established. 281 HTLV-1-infected patients from the HTLV reference center in Salvador, Brazil, were followed from 2005 to 2008. Patients were classified as asymptomatic, possible-, probable-, and definite-HAM/TSP, in accordance with diagnostic criteria proposed by De Castro-Costa et al. (2006): AIDS Res Hum Retroviruses 22:931–935. HTLV-1 proviral load was determined using real-time PCR. A receiver operator characteristic (ROC) curve was constructed using only asymptomatic individuals and definite-HAM/TSP patients. The ROC curve was used to predict the proviral load level that differentiates these two groups. Out of 281 patients, 189 were asymptomatic and 92 were diagnosed with HAM/TSP (22 possible, 23 probable, 47 definite). The mean HTLV-1 proviral load was higher in possible- (89,104 ± 93,006 copies/106 PBMC), -probable (175,854 ± 128,083 copies/106 PBMC), and definite-HAM/TSP patients (150,667 ± 122,320 copies/106 PBMC), when compared to asymptomatic individuals (27,178 ± 41,155 copies/106 PBMC) (P < 0.0001). A comparison of all HAM/TSP groups showed the highest proviral loads in probable-HAM/TSP patients, yet the differences in mean values were not statistically significant. The ROC curve suggested a value of 49,865 copies/106 PBMC, with 87% sensitivity (95% CI = 74–95) and 81% specificity (95% CI = 75–86), as the best proviral load cut-off point to differentiate definite HAM/TSP patients from asymptomatic individuals. HTLV-1 proviral loads are higher in groups of infected patients with neurological symptoms and may represent a relevant biological marker of disease progression.
Palavras-chave: HTLV-1
Proviral Load
Biomarker
HAM/TSP
Brazil
URI: http://www.repositorio.ufba.br/ri/handle/ri/3071
Data do documento: 2011
Aparece nas coleções:Artigo Publicado em Periódico Estrangeiro (ISC)

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