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Please use this identifier to cite or link to this item: http://repositorio.ufba.br/ri/handle/ri/25933

Title: Accelerated solvent extraction of phenolic compounds exploiting a Box-Behnken design and quantification of five flavonoids by HPLC-DAD in Passiflora species
Authors: Gomes, Silvana Vieira Floresta
Portugal, Lindomar Andrade
Anjos, Jeancarlo Pereira dos
Jesus, Onildo Nunes de
Oliveira, Eder Jorge de
David, Juceni Pereira de Lima
David, Jorge Mauricio
Issue Date: 4-May-2018
Abstract: This work describes the development of a method for the extraction of phenolic compounds, mainly flavonoids, from species of Passiflora by employing accelerated solvent extraction (ASE) and using a Box-Behnken design with desirability functions for optimization. The optimal extraction conditions consisted of an extraction temperature of 80 °C, 64% (w/w) ethanol and five number of extraction cycles. A high-performance liquid chromatography- diode array detection (HPLC-DAD) method for the identification and quantification of orientin, isoorientin, vitexin, isovitexin and rutin flavonoids in the leaves of seventeen Passiflora spp. using gradient elution with acetonitrile (solvent B) and 0.2% (w/w) formic acid in water (solvent A) as the eluent mixture was also developed and validated. These five flavonoids were quantified with good linearity, LOQs, LODs, precision, and accuracy. Higher concentrations of isoorientin, orientin, vitexin, isovitexin and rutin were determinate in P. edulis f. flavicarpa (1.61 mg/g extract and 0.58 mg/g dry plant), P. morifolia (2.10 mg/g extract and 0.90 mg/g dry plant), P. setacea (2.48 mg/g extract and 0.97 mg/g dry plant), P. setacea (8.46 mg/g extract and 3.30 mg/g dry plant) and P. galbana (3.48 mg/g extract and 1.02 mg/g dry plant), respectively.
URI: http://repositorio.ufba.br/ri/handle/ri/25933
ISSN: (0026-265X) Microchemical JournalL (Print)
Appears in Collections:Artigos Publicados em Periódicos (Renorbio)

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