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Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/17612
Tipo: Artigo de Periódico
Título: In vitro detection of hepatitis C virus in platelets from uninfected individuals exposed to the virus
Título(s) alternativo(s): Revista da Sociedade Brasileira de Medicina Tropical
Autor(es): Padovani, Juliana Lara
Corvino, Silvia Maria
Drexler, Jan Felix
Silva, Giovanni Faria
Pardini, Maria Inês de Moura Campos
Grotto, Rejane Maria Tommasini
Autor(es): Padovani, Juliana Lara
Corvino, Silvia Maria
Drexler, Jan Felix
Silva, Giovanni Faria
Pardini, Maria Inês de Moura Campos
Grotto, Rejane Maria Tommasini
Abstract: Introduction Despite hepatocytes being the target cells of hepatitis C virus (HCV), viral ribonucleic acid RNA has been detected in other cells, including platelets, which have been described as carriers of the virus in the circulation of infected patients. Platelets do not express cluster differentiation 81 CD81, the main receptor for the virus in hepatocytes, although this receptor protein has been found in megakaryocytes. Still, it is not clear if HCV interacts with platelets directly or if this interaction is a consequence of its association with megakaryocytes. The aim of this study was to evaluate the interaction of HCV with platelets from non-infected individuals, after in vitro exposure to the virus. Methods Platelets obtained from 50 blood donors not infected by HCV were incubated in vitro at 37°C for 48h with serum containing 100,000IU∕mL of genotype 1 HCV. After incubation, RNA extracted from the platelets was assayed for the presence of HCV by reverse transcription – polymerase chain reaction RT-PCR. Results After incubation in the presence of virus, all samples of platelets showed HCV RNA. Conclusions The results demonstrate that, in vitro, the virus interacts with platelets despite the absence of the receptor CD81, suggesting that other molecules could be involved in this association.
Palavras-chave: HCV
Platelets
CD81
País: Brasil
Tipo de Acesso: Acesso Aberto
URI: http://repositorio.ufba.br/ri/handle/ri/17612
Data do documento: 2013
Aparece nas coleções:Artigo Publicado em Periódico (Faculdade de Medicina)

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