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Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/17083
Tipo: Artigo de Periódico
Título: Selection of cryoprotectants based on their toxic effects on oyster gametes and embryos_6
Título(s) alternativo(s): Cryobiology
Autor(es): Nascimento, Iracema Andrade
Leite, Maria Bernadete Neiva Lemos
Araujo, Milena Maria Sampaio de
Sansone, Giovanni
Pereira, Solange Andrade
Santo, Maristela do Espírito
Autor(es): Nascimento, Iracema Andrade
Leite, Maria Bernadete Neiva Lemos
Araujo, Milena Maria Sampaio de
Sansone, Giovanni
Pereira, Solange Andrade
Santo, Maristela do Espírito
Abstract: Cryopreservation is a valuable tool for aquaculture by providing continuous seed production, regardless of the spawning seasons. This study aimed to select the least toxic among the cryoprotectants dimethyl sulfoxide (Me2SO), propylene glycol (PG), and methanol (MET) based on their toxicological effects on Crassostrea rhizophorae gametes and trochophores. They were exposed for 10, 20, and 30 min to a range of concentrations of those cryoprotectants. The endpoint was EC15–24 h (effective concentration which causes abnormalities in 15% of the population exposed to the cryoprotectants for 24 h), recently determined as the chronic value (the concentration at which chronic effects are first observed) for C. rhizophorae embryonic phases. There were no significant differences (p > 0.05) among the exposure times in Me2SO toxic effects to either gametes or trochophores. For MET, the increase in exposure time resulted in higher toxicity for gametes, but not for trochophores, while for PG there was a significant (p > 0.05) increase in toxicity with the increase of exposure for trochophores and spermatozoa, but not for oocytes. For gametes, MET was the most toxic among the cryoprotectants, while PG was the most toxic for trochophores.
Palavras-chave: Cryoprotectants
Toxic effects
Cryopreservation protocols
Oyster gametes
Trochophores
Tipo de Acesso: Acesso Aberto
URI: http://repositorio.ufba.br/ri/handle/ri/17083
Data do documento: 2005
Aparece nas coleções:Artigo Publicado em Periódico (LABIOMAR)

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