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Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/16685
Tipo: Artigo de Periódico
Título: Attenuated Nephritis in Inducible Nitric Oxide Synthase Knockout C57BL/6 Mice and Pulmonary Hemorrhage in CB17 SCID and Recombination Activating Gene 1 Knockout C57BL/6 Mice Infected with Leptospira interrogans
Título(s) alternativo(s): Infection and Immunity
Autor(es): Bandeira, Maurício de Sousa
Santos, Cleiton S.
Azevedo, Everton C. de
Soares, Luciane Marieta
Macedo, Júlio O.
Marchi, Samyra
Silva, Caroline Luane R. da
Chagas Júnior, Adenizar Delgado das
McBride, Alan J. A.
Reis, Mitermayer Galvão dos
McBride, Flávia W. C.
Athanazio, Daniel Abensur
Autor(es): Bandeira, Maurício de Sousa
Santos, Cleiton S.
Azevedo, Everton C. de
Soares, Luciane Marieta
Macedo, Júlio O.
Marchi, Samyra
Silva, Caroline Luane R. da
Chagas Júnior, Adenizar Delgado das
McBride, Alan J. A.
Reis, Mitermayer Galvão dos
McBride, Flávia W. C.
Athanazio, Daniel Abensur
Abstract: The aims of this study were to investigate the frequency of pulmonary hemorrhage (PH) in mice unable to produce functional B and T lymphocytes and to explore the effect of an inducible nitric oxide synthase gene (Inos) knockout (KO) on the frequency/severity of interstitial nephritis in vivo. We studied the outcome of infection by the virulent Leptospira interrogans serovar Copenhageni strain Cop. The animals used were Inos KO mice, recombination activating gene 1 (Rag1) KO mice, CB17 severe combined immunodeficiency (SCID) mice, and the respective wild-type (WT) C57BL/6 and BALB/c controls. The Inos KO and WT mice survived with no clinical symptoms of leptospirosis. The frequency and severity of nephritis was significantly lower in the Inos KO mice. All of the Rag1 KO and SCID animals died of acute leptospirosis, whereas all of the WT mice survived. PH was observed in 57 and 94% of Rag1 KO mice and in 83 and 100% of SCID mice, using inoculum doses of 107 and 106 leptospires, respectively. There was no evidence of PH in the WT controls. In conclusion, the loss of the Inos gene had a negligible effect on the outcome of leptospiral infection, although we observed a reduced susceptibility for interstitial nephritis in this group. Of note, the absence of functional B- and T-cell lymphocytes did not preclude the occurrence of PH. These data provide evidence that PH in leptospirosis may not be related only to autoimmune mechanisms.
Palavras-chave: Lung Diseases
Leptospira
Mice, SCID
Tipo de Acesso: Acesso Aberto
URI: http://repositorio.ufba.br/ri/handle/ri/16685
Data do documento: 2011
Aparece nas coleções:Artigo Publicado em Periódico (Faculdade de Medicina)

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