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Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/15912
Tipo: Artigo de Periódico
Título: Leishmania amazonensis infection impairs differentiation and function of human dendritic cells
Título(s) alternativo(s): Journal of Leukocyte Biology
Autor(es): Favali, Cecilia Beatriz Fiuza
Tavares, Natália Machado
Clarêncio, Jorge
Barral, Aldina Maria Prado
Barral-Netto, Manoel
Brodskyn, Claudia Ida
Autor(es): Favali, Cecilia Beatriz Fiuza
Tavares, Natália Machado
Clarêncio, Jorge
Barral, Aldina Maria Prado
Barral-Netto, Manoel
Brodskyn, Claudia Ida
Abstract: Dendritic cells (DCs) are of utmost importance in initiating an immune response and may also function as targets for pathogens. The presence of pathogens inside DCs is likely to impair their functions and thus, influence immune responses. In the present report, we evaluated the impact of the presence of Leishmania amazonensis during differentiation and maturation of human monocyte-derived DCs. The presence of live L. amazonensis parasites during DC differentiation led to a significant decrease in CD80 (92%) and CD1a (56%) expression and an increase in CD86 (56%) cell surface expression. Phenotypic changes were accompanied by a lower secretion of IL-6, observed after 6 days of DC differentiation in the presence of L. amazonensis. DCs differentiated in the presence of L. amazonensis were used as APC in an autologous coculture, and lower amounts of IFN-γ were obtained compared with control DCs differentiated in the absence of parasites. The effect of heat-killed parasites, but not of Leishmania antigen, during DC differentiation and maturation was similar to that observed with viable parasites. During maturation, the presence of live L. amazonensis parasites, but not of soluble Leishmania antigen, led to a decrease in IL-6 and IL-10 production. In this way, we observed that the parasite is able to abrogate full DC differentiation, causing a delay in the immune response and likely, favoring its establishment in human hosts.
Palavras-chave: Leishmaniasis
Antigen-presenting cells
Cytokines
Costimulatory molecules
T cells
Tipo de Acesso: Acesso Aberto
URI: http://repositorio.ufba.br/ri/handle/ri/15912
Data do documento: 2007
Aparece nas coleções:Artigo Publicado em Periódico (Faculdade de Medicina)

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