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|Title: ||Administration of granulocyte colony-stimulating factor induces immunomodulation, recruitment of T regulatory cells, reduction of myocarditis and decrease of parasite load in a mouse model of chronic Chagas disease cardiomyopathy|
|Other Titles: ||FASEB Journal|
|Authors: ||Vasconcelos, Juliana Fraga|
Souza, Bruno Solano de Freitas
Lins, Thayse F. S.
Garcia, Letícia M. S.
Kaneto, Carla Martins
Sampaio, Geraldo P.
Alcântara, Adriano Costa de
Alcântara, Adriano Costa de
Meira, Cássio S.
Macambira, Simone Garcia
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
|Keywords: ||Trypanosoma cruzi;Cytokine therapy;Inflammation;Fibrosis;Th1 modulation|
|Issue Date: ||2013|
|Abstract: ||Chagas disease, caused by Trypanosoma cruzi infection, is a leading cause of heart failure in Latin American countries. In a previous study, we showed beneficial effects of granulocyte colony-stimulating factor (G-CSF) administration in the heart function of mice with chronic T. cruzi infection. Presently, we investigated the mechanisms by which this cytokine exerts its beneficial effects. Mice chronically infected with T. cruzi were treated with human recombinant G-CSF (3 courses of 200 μg/kg/d for 5 d). Inflammation and fibrosis were reduced in the hearts of G-CSF-treated mice, compared with the hearts of vehicle-treated mice, which correlated with decreased syndecan-4, intercellular adhesion molecule-1, and galectin-3 expressions. Marked reductions in interferon-γ and tumor necrosis factor-α and increased interleukin-10 and transforming growth factor-β were found after G-CSF administration. Because the therapy did not induce a Th1 to Th2 immune response deviation, we investigated the role of regulatory T (Treg) cells. A significant increase in CD3+Foxp3+ cells was observed in the hearts of G-CSF-treated mice. In addition, a reduction of parasitism was observed after G-CSF treatment. Our results indicate a role of Treg cells in the immunosuppression induced by G-CSF treatment and reinforces its potential therapeutic use for patients with Chagas disease.—Vasconcelos, J. F., Souza, B. S. F., Lins, T. F. S., Garcia, L. M. S., Kaneto, C. M., Smapaio, G. P., de Alcântara, A. C., Meira, C. S., Macambira, S. G., Ribeiro-dos-Santos, R., Soares, M. B. P. Administration of granulocyte colony-stimulating factor induces immunomodulation, recruitment of T regulatory cells, reduction of myocarditis and decrease of parasite load in a mouse model of chronic Chagas disease cardiomyopathy.|
|Description: ||Texto completo: acesso restrito. p. 4691-4702|
|Appears in Collections:||Artigos Publicados em Periódicos (EMV)|
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