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Please use this identifier to cite or link to this item: http://repositorio.ufba.br/ri/handle/ri/14844

Title: Viability of Sporulated Oocysts of Neospora caninum After Exposure to Different Physical and Chemical Treatments
Other Titles: Journal of Parasitology
Authors: Nishi, Sandra Mayumi
Soares, Rodrigo Martins
Driemeier, David
Antoniassi, Nadia Aline Bobbi
Schares, Gereon
Gennari, Solange Maria
Alves Neto, Aldo Francisco
Issue Date: 2011
Abstract: The aim of the present study was to evaluate the viability of Neospora caninum sporulated oocysts after various chemical and physical treatments. Bioassays in gerbils and molecular techniques (PCR-RFLP) were used for identification of the oocysts shed by experimentally infected dogs. Sporulated oocysts were purified and divided into 11 treatment groups as follows: absolute ethanol for 1 hr; 20 C for 6 hr; 4 C for 6 hr; 60 C for 1 min; 100 C for 1 min; 10% formaldehyde for 1 hr; 10% ammonia for 1 hr; 2% iodine for 1 hr; 10% sodium hypochlorite for 1 hr; 70% ethanol for 1 hr; and one group was left untreated and kept as a positive control. All chemical treatments were performed at room temperature (37 C). A total of 33 gerbils, or 3 gerbils per treatment, were used for bioassays. After treatment, the oocysts were divided into aliquots of 1,000 oocysts and orally administered to gerbils. After 63 days, the gerbils were anesthetized and killed with 0.2 ml of T61; blood and tissue samples were collected for serological (IFAT and western blotting), molecular (real-time PCR), histopathology, and immunohistochemical tests. Treatments were considered effective only if all 5 detection techniques tested negative. High temperatures at 100 C for 1 min and 10% sodium hypochlorite for 1 hr were the only treatments that met this condition, effectively inactivating all oocysts.
Description: p. 135-139
URI: http://repositorio.ufba.br/ri/handle/ri/14844
ISSN: 0022-3395
Appears in Collections:Artigos Publicados em Periódicos (EMV)

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